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PTEN Recombinant antibody, PBS Only

PTEN Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240134F7

Cat no : 80718-7-PBS

Print datasheet

Synonyms

10q23del, BZS, MHAM, MMAC1, phosphatase and tensin homolog, PTEN, PTEN1, TEP1

验证数据展示

  • IF Staining of HeLa using 80718-7-RR (same clone as 80718-7-PBS)

    IF Staining of HeLa using 80718-7-RR (same clone as 80718-7-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PTEN antibody (80718-7-RR, Clone: 240134F7 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80718-7-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 80718-7-RR (same clone as 80718-7-PBS)

    FC experiment of HeLa using 80718-7-RR (same clone as 80718-7-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug PTEN Recombinant antibody (80718-7-RR, Clone:240134F7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 80718-7-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80718-7-PBS

    Affinity and Kinetic Characterization of 80718-7-PBS

    Biolayer interferometry (BLl) kinetic assays of 80718-7-RR against Human PTEN were performed. The affinity constant is below 1 pM.

Planning an IHC experiment? We recommend our IHCeasy PTEN Ready-To-Use IHC Kit. PTEN primary antibody included.

产品信息

80718-7-PBS targets PTEN in IF/ICC, FC (Intra), ELISA applications and shows reactivity with Human samples.

Tested Applications IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity Human
Immunogen PTEN fusion protein Ag17274 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name phosphatase and tensin homolog
Synonyms 10q23del, BZS, MHAM, MMAC1, phosphatase and tensin homolog, PTEN, PTEN1, TEP1
Calculated Molecular Weight 47 kDa
GenBank Accession NumberBC005821
Gene Symbol PTEN
Gene ID (NCBI) 5728
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP60484
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C