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CoraLite®594-conjugated PTEN Monoclonal antibody

PTEN Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

5C10B6

Cat No : CL594-60300

Print datasheet

Synonyms

10q23del, BZS, MHAM, MMAC1, phosphatase and tensin homolog, PTEN, PTEN1, TEP1

验证数据展示

  • IF Staining of HepG2 using CL594-60300

    IF Staining of HepG2 using CL594-60300

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL594-60300 (PTEN antibody) at dilution of 1:100.

  • FC experiment of HepG2 using CL594-60300

    FC experiment of HepG2 using CL594-60300

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human PTEN (CL594-60300, Clone:5C10B6) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PTEN Ready-To-Use IHC Kit. PTEN primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60300-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-60300 targets PTEN in IF, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity human, mouse
Immunogen PTEN fusion protein Ag17274 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name phosphatase and tensin homolog
Synonyms 10q23del, BZS, MHAM, MMAC1, phosphatase and tensin homolog, PTEN, PTEN1, TEP1
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC005821
Gene Symbol PTEN
Gene ID (NCBI) 5728
RRIDAB_2883458
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP60484
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PTEN (also designated MMAC1), products of tumor suppressor genes, are found deleted in most human gliomas. The PTEN genes are also mutated in many other tumors, such as brain, breast, kidney and prostate cancers. PTEN is a protein tyrosine phosphatase that may terminate the signaling transduction pathways mediated by PI 3-kinase/Akt. PTEN has an apparent molecular weight of 55 kDa and it is located in the cytosol.

实验方案

Product Specific Protocols
IF protocol for CL594 PTEN antibody CL594-60300Download protocol
Standard Protocols
Click here to view our Standard Protocols