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验证数据展示

  • IF Staining of HepG2 using CL594-60300

    IF Staining of HepG2 using CL594-60300

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL594-60300 (PTEN antibody) at dilution of 1:100.

  • FC experiment of HepG2 using CL594-60300

    FC experiment of HepG2 using CL594-60300

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human PTEN (CL594-60300, Clone:5C10B6) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated PTEN Monoclonal antibody

PTEN Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-60300

产品说明书

CloneNo. 5C10B6

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse

应用

IF/ICC, FC (Intra)

别名

10q23del, 5C10B6, Bannayan Zonana syndrome, BC005821, BZS

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PTEN Ready-To-Use IHC Kit. PTEN primary antibody included.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-60300 targets PTEN in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 PTEN fusion protein Ag17274 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 phosphatase and tensin homolog
别名 10q23del, 5C10B6, Bannayan Zonana syndrome, BC005821, BZS
计算分子量 47 kDa
观测分子量 55 kDa
GenBank蛋白编号BC005821
基因名称 PTEN
Gene ID (NCBI) 5728
RRIDAB_2883458
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP60484
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PTEN (also designated MMAC1), products of tumor suppressor genes, are found deleted in most human gliomas. The PTEN genes are also mutated in many other tumors, such as brain, breast, kidney and prostate cancers. PTEN is a protein tyrosine phosphatase that may terminate the signaling transduction pathways mediated by PI 3-kinase/Akt. PTEN has an apparent molecular weight of 55 kDa.

实验方案

Product Specific Protocols
IF protocol for CL594 PTEN antibody CL594-60300Download protocol
Standard Protocols
Click here to view our Standard Protocols