CoraLite®555-conjugated SHP2 Monoclonal antibody
SHP2 Monoclonal Antibody for IF/ICC, FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite®555 Fluorescent Dye
CloneNo.
3F8A8
验证数据展示
经过测试的应用
Positive IF/ICC detected in | MCF-7 cells |
Positive FC (Intra) detected in | MCF-7 cells |
Planning an IHC experiment? We recommend our IHCeasy PTPN11 Ready-To-Use IHC Kit. PTPN11 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66795-1-Ig
推荐稀释比
Application | Dilution |
---|---|
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL555-66795 targets SHP2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF/ICC, FC (Intra) Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | SHP2 fusion protein Ag13649 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | protein tyrosine phosphatase, non-receptor type 11 |
Synonyms | PTPN11/SHP2, PTPN11, NS1, NS 1, EC:3.1.3.48 |
Calculated Molecular Weight | 597 aa, 68 kDa |
Observed Molecular Weight | 68 kDa |
GenBank Accession Number | BC008692 |
Gene Symbol | PTPN11 |
Gene ID (NCBI) | 5781 |
RRID | AB_2919695 |
Conjugate | CoraLite®555 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 557 nm / 570 nm |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | Q06124 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PTPN11 (protein tyrosine phosphatase, non-receptor type 11) is also named as PTP-1D, PTP2, PTP2C, PTP3, SHP2, CFC, CFC, BPTP3, SH-PTP2, SH-PTP3, MGC14433 and belongs to the protein-tyrosine phosphatase family and non-receptor class 2 subfamily. It modulates and regulates signaling through numerous pathways, many of which are active in the developing endocardial cushions and implicated the ERK pathway as a central mechanism (PMID: 19017799). Its signaling may play equally important roles in retinal survival in both physiological and pathological conditions (PMID: 21576358). Defects in PTPN11 are the cause of LEOPARD syndrome type 1 (LEOPARD1), Noonan syndrome type 1 (NS1), juvenile myelomonocytic leukemia (JMML) and metachondromatosis (MC). It has 3 isoforms produced by alternative splicing.
实验方案
Product Specific Protocols | |
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IF protocol for CL555 SHP2 antibody CL555-66795 | Download protocol |
FC protocol for CL555 SHP2 antibody CL555-66795 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |