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PTPRO Monoclonal antibody, PBS Only

PTPRO Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Pig

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

2F2B4

Cat No : 67000-1-PBS

Print datasheet

Synonyms

GLEPP1, PTP U2, PTPase U2, PTPRO, PTPU2, R PTP O

验证数据展示

  • IF Staining of human kidney using 67000-1-Ig (same clone as 67000-1-PBS)

    IF Staining of human kidney using 67000-1-Ig (same clone as 67000-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using PTPRO antibody (67000-1-Ig, Clone: 2F2B4 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), ACE2 antibody (21115-1-AP, green). This data was developed using the same antibody clone with 67000-1-PBS in a different storage buffer formulation.

  • IF Staining of human kidney using 67000-1-Ig (same clone as 67000-1-PBS)

    IF Staining of human kidney using 67000-1-Ig (same clone as 67000-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using PTPRO antibody (67000-1-Ig, Clone: 2F2B4 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), ACE2 antibody (21115-1-AP, green). This data was developed using the same antibody clone with 67000-1-PBS in a different storage buffer formulation.

  • WB analysis of pig kidney using 67000-1-Ig (same clone as 67000-1-PBS)

    WB analysis of pig kidney using 67000-1-Ig (same clone as 67000-1-PBS)

    pig kidney tissue were subjected to SDS PAGE followed by western blot with 67000-1-Ig (PTPRO antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67000-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 67000-1-Ig (same clone as 67000-1-PBS)

    IHC staining of human kidney using 67000-1-Ig (same clone as 67000-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67000-1-Ig (PTPRO antibody) at dilution of 1:3000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67000-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PTPRO antibody included
  • IHC staining of human kidney using 67000-1-Ig (same clone as 67000-1-PBS)

    IHC staining of human kidney using 67000-1-Ig (same clone as 67000-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67000-1-Ig (PTPRO antibody) at dilution of 1:3000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67000-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PTPRO antibody included
Planning an IHC experiment? We recommend our IHCeasy PTPRO Ready-To-Use IHC Kit. PTPRO primary antibody included.

产品信息

67000-1-PBS targets PTPRO in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Pig samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity Human, Pig
Immunogen PTPRO fusion protein Ag8284 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name protein tyrosine phosphatase, receptor type, O
Synonyms GLEPP1, PTP U2, PTPase U2, PTPRO, PTPU2, R PTP O
Calculated Molecular Weight 138 kDa
Observed Molecular Weight 160 kDa, 180-220 kDa
GenBank Accession NumberBC035960
Gene Symbol PTPRO
Gene ID (NCBI) 5800
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16827
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

PTPRO(receptor-type tyrosine-protein phosphatase O) is also named as GLEPP1, PTPU2 and belongs to the protein-tyrosine phosphatase family. The protein is a receptor-like membrane protein tyrosine phosphatase expressed at the apical membrane of the podocyte foot processes in the kidney(PMID:21722858). The 1,159-amino acid predicted mature protein contains a large extracellular domain, a single transmembrane domain, and a single intracellular PTPase domain. Defects in PTPRO are the cause of nephrotic syndrome type 6 (NPHS6). In reducing conditions, PTPRO can form a smear from 180 to 220 kDa; In non-reducing conditions, a band appeares around 350 kDa, which likely represents the dimeric form of full-length PTPRO (PMID: 19573017).