• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IF Staining of human kidney using 67000-1-Ig (same clone as 67000-1-PBS)

    IF Staining of human kidney using 67000-1-Ig (same clone as 67000-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using PTPRO antibody (67000-1-Ig, Clone: 2F2B4 ) at dilution of 1:800 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L), ACE2 antibody (21115-1-AP, green). This data was developed using the same antibody clone with 67000-1-PBS in a different storage buffer formulation.

  • IF Staining of human kidney using 67000-1-Ig (same clone as 67000-1-PBS)

    IF Staining of human kidney using 67000-1-Ig (same clone as 67000-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using PTPRO antibody (67000-1-Ig, Clone: 2F2B4 ) at dilution of 1:800 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L), ACE2 antibody (21115-1-AP, green). This data was developed using the same antibody clone with 67000-1-PBS in a different storage buffer formulation.

  • WB analysis of pig kidney using 67000-1-Ig (same clone as 67000-1-PBS)

    WB analysis of pig kidney using 67000-1-Ig (same clone as 67000-1-PBS)

    pig kidney tissue were subjected to SDS PAGE followed by western blot with 67000-1-Ig (PTPRO antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67000-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 67000-1-Ig (same clone as 67000-1-PBS)

    IHC staining of human kidney using 67000-1-Ig (same clone as 67000-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67000-1-Ig (PTPRO antibody) at dilution of 1:3000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67000-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PTPRO antibody included
  • IHC staining of human kidney using 67000-1-Ig (same clone as 67000-1-PBS)

    IHC staining of human kidney using 67000-1-Ig (same clone as 67000-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67000-1-Ig (PTPRO antibody) at dilution of 1:3000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67000-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PTPRO antibody included

PTPRO Monoclonal antibody, PBS Only

PTPRO Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 67000-1-PBS

产品说明书

CloneNo. 2F2B4

宿主/亚型

Mouse / IgG2b

种属反应性

Human, Pig

应用

WB, IHC, IF-P, Indirect ELISA

别名

GLEPP1, PTP U2, PTPase U2, PTPRO, PTPU2, R PTP O

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy PTPRO Ready-To-Use IHC Kit. PTPRO primary antibody included.

产品信息

67000-1-PBS targets PTPRO in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, Pig samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 Human, Pig
免疫原 PTPRO fusion protein Ag8284 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 protein tyrosine phosphatase, receptor type, O
别名 GLEPP1, PTP U2, PTPase U2, PTPRO, PTPU2, R PTP O
计算分子量 138 kDa
观测分子量 160 kDa, 180-220 kDa
GenBank蛋白编号BC035960
基因名称 PTPRO
Gene ID (NCBI) 5800
RRIDAB_2882317
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ16827
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PTPRO(receptor-type tyrosine-protein phosphatase O) is also named as GLEPP1, PTPU2 and belongs to the protein-tyrosine phosphatase family. The protein is a receptor-like membrane protein tyrosine phosphatase expressed at the apical membrane of the podocyte foot processes in the kidney(PMID:21722858). The 1,159-amino acid predicted mature protein contains a large extracellular domain, a single transmembrane domain, and a single intracellular PTPase domain. Defects in PTPRO are the cause of nephrotic syndrome type 6 (NPHS6). In reducing conditions, PTPRO can form a smear from 180 to 220 kDa; In non-reducing conditions, a band appeares around 350 kDa, which likely represents the dimeric form of full-length PTPRO (PMID: 19573017).