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PTPRO Monoclonal antibody

PTPRO Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Pig and More (1)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2F2B4

Cat no : 67000-1-Ig

Print datasheet

Synonyms

GLEPP1, PTP U2, PTPase U2, PTPRO, PTPU2, R PTP O

验证数据展示

  • IF Staining of human kidney using 67000-1-Ig

    IF Staining of human kidney using 67000-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using PTPRO antibody (67000-1-Ig, Clone: 2F2B4 ) at dilution of 1:800 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L), ACE2 antibody (21115-1-AP, green).

  • IF Staining of human kidney using 67000-1-Ig

    IF Staining of human kidney using 67000-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using PTPRO antibody (67000-1-Ig, Clone: 2F2B4 ) at dilution of 1:800 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L), ACE2 antibody (21115-1-AP, green).

  • WB analysis of pig kidney using 67000-1-Ig

    WB analysis of pig kidney using 67000-1-Ig

    pig kidney tissue were subjected to SDS PAGE followed by western blot with 67000-1-Ig (PTPRO antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 67000-1-Ig

    IHC staining of human kidney using 67000-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67000-1-Ig (PTPRO antibody) at dilution of 1:3000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PTPRO antibody included
  • IHC staining of human kidney using 67000-1-Ig

    IHC staining of human kidney using 67000-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67000-1-Ig (PTPRO antibody) at dilution of 1:3000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PTPRO antibody included

经过测试的应用

Positive WB detected inpig kidney tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman kidney tissue
Planning an IHC experiment? We recommend our IHCeasy PTPRO Ready-To-Use IHC Kit. PTPRO primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

67000-1-Ig targets PTPRO in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Pig samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human, Pig
Cited Reactivityhuman
Immunogen PTPRO fusion protein Ag8284 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name protein tyrosine phosphatase, receptor type, O
Synonyms GLEPP1, PTP U2, PTPase U2, PTPRO, PTPU2, R PTP O
Calculated Molecular Weight 138 kDa
Observed Molecular Weight 160 kDa, 180-220 kDa
GenBank Accession NumberBC035960
Gene Symbol PTPRO
Gene ID (NCBI) 5800
RRIDAB_2882317
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16827
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

PTPRO(receptor-type tyrosine-protein phosphatase O) is also named as GLEPP1, PTPU2 and belongs to the protein-tyrosine phosphatase family. The protein is a receptor-like membrane protein tyrosine phosphatase expressed at the apical membrane of the podocyte foot processes in the kidney(PMID:21722858). The 1,159-amino acid predicted mature protein contains a large extracellular domain, a single transmembrane domain, and a single intracellular PTPase domain. Defects in PTPRO are the cause of nephrotic syndrome type 6 (NPHS6). In reducing conditions, PTPRO can form a smear from 180 to 220 kDa; In non-reducing conditions, a band appeares around 350 kDa, which likely represents the dimeric form of full-length PTPRO (PMID: 19573017).

实验方案

Product Specific Protocols
WB protocol for PTPRO antibody 67000-1-IgDownload protocol
IHC protocol for PTPRO antibody 67000-1-IgDownload protocol
IF protocol for PTPRO antibody 67000-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mediators Inflamm

MiR-6803-5p Promotes Cancer Cell Proliferation and Invasion via PTPRO/NF-κB Axis in Colorectal Cancer.

Authors - Shushan Yan
View Article