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CoraLite® Plus 488-conjugated PTPRO Monoclonal antibody

PTPRO Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Pig

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2F2B4

Cat no : CL488-67000

Print datasheet

Synonyms

GLEPP1, PTP U2, PTPase U2, PTPRO, PTPU2, R PTP O

验证数据展示

  • IF Staining of human kidney using CL488-67000

    IF Staining of human kidney using CL488-67000

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using CoraLite® Plus 488 PTPRO antibody (CL488-67000, Clone: 2F2B4 ) at dilution of 1:200.

经过测试的应用

Positive IF-P detected inhuman kidney tissue
Planning an IHC experiment? We recommend our IHCeasy PTPRO Ready-To-Use IHC Kit. PTPRO primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67000-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67000 targets PTPRO in IF-P applications and shows reactivity with Human, Pig samples.

Tested Applications IF-P Application Description
Tested Reactivity Human, Pig
Immunogen PTPRO fusion protein Ag8284 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name protein tyrosine phosphatase, receptor type, O
Synonyms GLEPP1, PTP U2, PTPase U2, PTPRO, PTPU2, R PTP O
Calculated Molecular Weight 138 kDa
Observed Molecular Weight 160 kDa, 180-220 kDa
GenBank Accession NumberBC035960
Gene Symbol PTPRO
Gene ID (NCBI) 5800
RRIDAB_2919402
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16827
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PTPRO(receptor-type tyrosine-protein phosphatase O) is also named as GLEPP1, PTPU2 and belongs to the protein-tyrosine phosphatase family. The protein is a receptor-like membrane protein tyrosine phosphatase expressed at the apical membrane of the podocyte foot processes in the kidney(PMID:21722858). The 1,159-amino acid predicted mature protein contains a large extracellular domain, a single transmembrane domain, and a single intracellular PTPase domain. Defects in PTPRO are the cause of nephrotic syndrome type 6 (NPHS6). In reducing conditions, PTPRO can form a smear from 180 to 220 kDa; In non-reducing conditions, a band appeares around 350 kDa, which likely represents the dimeric form of full-length PTPRO (PMID: 19573017).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PTPRO antibody CL488-67000Download protocol
Standard Protocols
Click here to view our Standard Protocols