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验证数据展示

  • IF Staining of human kidney using CL594-67000

    IF Staining of human kidney using CL594-67000

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using CoraLite®594 PTPRO antibody (CL594-67000, Clone: 2F2B4 ) at dilution of 1:100.

CoraLite®594-conjugated PTPRO Monoclonal antibody

PTPRO Monoclonal Antibody for IF-P
Cat No. CL594-67000

产品说明书

CloneNo. 2F2B4

宿主/亚型

Mouse / IgG2b

种属反应性

Human, Pig

应用

IF-P

别名

GLEPP1, PTP U2, PTPase U2, PTPRO, PTPU2, R PTP O

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman kidney tissue
Planning an IHC experiment? We recommend our IHCeasy PTPRO Ready-To-Use IHC Kit. PTPRO primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67000-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67000 targets PTPRO in IF-P applications and shows reactivity with Human, Pig samples.

经测试应用 IF-P Application Description
经测试反应性 Human, Pig
免疫原 PTPRO fusion protein Ag8284 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 protein tyrosine phosphatase, receptor type, O
别名 GLEPP1, PTP U2, PTPase U2, PTPRO, PTPU2, R PTP O
计算分子量 138 kDa
观测分子量 160 kDa, 180-220 kDa
GenBank蛋白编号BC035960
基因名称 PTPRO
Gene ID (NCBI) 5800
RRIDAB_2920069
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ16827
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PTPRO(receptor-type tyrosine-protein phosphatase O) is also named as GLEPP1, PTPU2 and belongs to the protein-tyrosine phosphatase family. The protein is a receptor-like membrane protein tyrosine phosphatase expressed at the apical membrane of the podocyte foot processes in the kidney(PMID:21722858). The 1,159-amino acid predicted mature protein contains a large extracellular domain, a single transmembrane domain, and a single intracellular PTPase domain. Defects in PTPRO are the cause of nephrotic syndrome type 6 (NPHS6). In reducing conditions, PTPRO can form a smear from 180 to 220 kDa; In non-reducing conditions, a band appeares around 350 kDa, which likely represents the dimeric form of full-length PTPRO (PMID: 19573017).

实验方案

Product Specific Protocols
IF protocol for CL594 PTPRO antibody CL594-67000Download protocol
Standard Protocols
Click here to view our Standard Protocols