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验证数据展示

  • IF Staining of HepG2 using 83981-4-RR (same clone as 83981-4-PBS)

    IF Staining of HepG2 using 83981-4-RR (same clone as 83981-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Securin antibody (83981-4-RR, Clone: 241090G10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83981-4-PBS in a different storage buffer formulation.

  • FC experiment of Jurkat using 83981-4-RR (same clone as 83981-4-PBS)

    FC experiment of Jurkat using 83981-4-RR (same clone as 83981-4-PBS)

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug Securin Recombinant antibody (83981-4-RR, Clone:241090G10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83981-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00876-1

    Cytometric bead array standard curve of MP00876-1

    Cytometric bead array standard curve of MP00876-1, Securin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83981-2-PBS. Detection antibody: 83981-4-PBS. Standard: Ag12670. Range: 0.78-100 ng/mL.

  • Cytometric bead array standard curve of MP00876-2

    Cytometric bead array standard curve of MP00876-2

    Cytometric bead array standard curve of MP00876-2, Securin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83981-1-PBS. Detection antibody: 83981-4-PBS. Standard: Ag12670. Range: 1.56-100 ng/mL.

  • Cytometric bead array standard curve of MP00876-3

    Cytometric bead array standard curve of MP00876-3

    Cytometric bead array standard curve of MP00876-3, Securin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83981-4-PBS. Detection antibody: 83981-3-PBS. Standard: Ag12670. Range: 1.56-100 ng/mL.

  • Affinity and Kinetic Characterization of 83981-4-RR

    Affinity and Kinetic Characterization of 83981-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83981-4-RR against Human Securin were performed. The affinity constant is 0.155 nM.

Securin Recombinant antibody, PBS Only (Capture/Detector)

Securin Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 83981-4-PBS

产品说明书

CloneNo. 241090G10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

PTTG1, PTTG, Pituitary tumor-transforming gene 1 protein, hPTTG, Esp1-associated protein

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PTTG1/Securin Ready-To-Use IHC Kit. PTTG1/Securin primary antibody included.

产品信息

83981-4-PBS targets Securin as part of a matched antibody pair:

MP00876-1: 83981-2-PBS capture and 83981-4-PBS detection (validated in Cytometric bead array)

MP00876-2: 83981-1-PBS capture and 83981-4-PBS detection (validated in Cytometric bead array)

MP00876-3: 83981-4-PBS capture and 83981-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 Securin fusion protein Ag12670 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 pituitary tumor-transforming 1
别名 PTTG1, PTTG, Pituitary tumor-transforming gene 1 protein, hPTTG, Esp1-associated protein
计算分子量 202 aa, 22 kDa
GenBank蛋白编号BC101834
基因名称 Securin
Gene ID (NCBI) 9232
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO95997
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Securin, also known as the pituitary tumor transforming gene (PTTG1), was first isolated from rat pituitary tumor cells. Securin is a multi-functional protein that regulates sister chromatin segregation in mitosis, DNA repair, gene transcription, metabolism, and organ development. Securin is normally expressed in tissues with high proliferative activity, including the spleen, thymus gland, and testis, whereas it is rarely expressed in differentiated mature tissues (PMID: 22563433, PMID: 36123907). The calculated molecular weight of Securin is 22 kDa. With post-translational modification, the molecular weight of Securin will be migrated to 28 kDa.