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验证数据展示

  • IF Staining of HepG2 using 83981-4-RR

    IF Staining of HepG2 using 83981-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Securin antibody (83981-4-RR, Clone: 241090G10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of Jurkat using 83981-4-RR

    FC experiment of Jurkat using 83981-4-RR

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug Securin Recombinant antibody (83981-4-RR, Clone:241090G10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83981-4-RR

    Affinity and Kinetic Characterization of 83981-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83981-4-RR against Human Securin were performed. The affinity constant is 0.155 nM.

Securin Recombinant antibody

Securin Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83981-4-RR

产品说明书

CloneNo. 241090G10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA

别名

PTTG1, PTTG, Pituitary tumor-transforming gene 1 protein, hPTTG, Esp1-associated protein

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inJurkat cells, A431 cells
Planning an IHC experiment? We recommend our IHCeasy PTTG1/Securin Ready-To-Use IHC Kit. PTTG1/Securin primary antibody included.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83981-4-RR targets Securin in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 Securin fusion protein Ag12670 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 pituitary tumor-transforming 1
别名 PTTG1, PTTG, Pituitary tumor-transforming gene 1 protein, hPTTG, Esp1-associated protein
计算分子量 202 aa, 22 kDa
GenBank蛋白编号BC101834
基因名称 Securin
Gene ID (NCBI) 9232
RRIDAB_3671555
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO95997
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Securin, also known as the pituitary tumor transforming gene (PTTG1), was first isolated from rat pituitary tumor cells. Securin is a multi-functional protein that regulates sister chromatin segregation in mitosis, DNA repair, gene transcription, metabolism, and organ development. Securin is normally expressed in tissues with high proliferative activity, including the spleen, thymus gland, and testis, whereas it is rarely expressed in differentiated mature tissues (PMID: 22563433, PMID: 36123907). The calculated molecular weight of Securin is 22 kDa. With post-translational modification, the molecular weight of Securin will be migrated to 28 kDa.

实验方案

Product Specific Protocols
IF protocol for Securin antibody 83981-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols