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验证数据展示

  • IF Staining of HepG2 using CL488-83981-4

    IF Staining of HepG2 using CL488-83981-4

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 Securin antibody (CL488-83981-4, Clone: 241090G10 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated Securin Recombinant antibody

Securin Recombinant Antibody for IF/ICC
Cat No. CL488-83981-4

Print datasheet

CloneNo. 241090G10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PTTG1/Securin Ready-To-Use IHC Kit. PTTG1/Securin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 83981-4-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-83981-4 targets Securin in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen Securin fusion protein Ag12670 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name pituitary tumor-transforming 1
Synonyms
Calculated Molecular Weight 202 aa, 22 kDa
GenBank Accession NumberBC101834
Gene Symbol Securin
Gene ID (NCBI) 9232
RRIDAB_3673330
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO95997
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Securin, also known as the pituitary tumor transforming gene (PTTG1), was first isolated from rat pituitary tumor cells. Securin is a multi-functional protein that regulates sister chromatin segregation in mitosis, DNA repair, gene transcription, metabolism, and organ development. Securin is normally expressed in tissues with high proliferative activity, including the spleen, thymus gland, and testis, whereas it is rarely expressed in differentiated mature tissues (PMID: 22563433, PMID: 36123907). The calculated molecular weight of Securin is 22 kDa. With post-translational modification, the molecular weight of Securin will be migrated to 28 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Securin antibody CL488-83981-4Download protocol
Standard Protocols
Click here to view our Standard Protocols