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验证数据展示

  • IF Staining of HepG2 using CL488-83981-4

    IF Staining of HepG2 using CL488-83981-4

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 Securin antibody (CL488-83981-4, Clone: 241090G10 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated Securin Recombinant antibody

Securin Recombinant Antibody for IF/ICC
Cat No. CL488-83981-4

产品说明书

CloneNo. 241090G10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC

别名

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PTTG1/Securin Ready-To-Use IHC Kit. PTTG1/Securin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 83981-4-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-83981-4 targets Securin in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 Securin fusion protein Ag12670 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 pituitary tumor-transforming 1
别名
计算分子量 202 aa, 22 kDa
GenBank蛋白编号BC101834
基因名称 Securin
Gene ID (NCBI) 9232
RRIDAB_3673330
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO95997
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Securin, also known as the pituitary tumor transforming gene (PTTG1), was first isolated from rat pituitary tumor cells. Securin is a multi-functional protein that regulates sister chromatin segregation in mitosis, DNA repair, gene transcription, metabolism, and organ development. Securin is normally expressed in tissues with high proliferative activity, including the spleen, thymus gland, and testis, whereas it is rarely expressed in differentiated mature tissues (PMID: 22563433, PMID: 36123907). The calculated molecular weight of Securin is 22 kDa. With post-translational modification, the molecular weight of Securin will be migrated to 28 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Securin antibody CL488-83981-4Download protocol
Standard Protocols
Click here to view our Standard Protocols