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验证数据展示

  • IF Staining of MCF-7 using CL488-80084

    IF Staining of MCF-7 using CL488-80084

    Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated MCF-7 cells using CoraLite® Plus 488 Phospho-Beta Catenin (Ser675) antibody (CL488-80084, Clone: 6D6 ) at dilution of 1:200, CL594-Phalloidin (red).

CoraLite® Plus 488-conjugated Phospho-Beta Catenin (Ser675) Recombinant antibody

Phospho-Beta Catenin (Ser675) Uni-rAbTM Recombinant Antibody for IF/ICC
Cat No. CL488-80084

产品说明书

CloneNo. 6D6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC

别名

Beta Catenin (Ser675), p Beta Catenin, p Beta Catenin (Ser675), p Beta Catenin Ser675, p-Beta Catenin

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inλ phosphatase treated MCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy Beta Catenin Ready-To-Use IHC Kit. Beta Catenin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 80084-1-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-80084 targets Phospho-Beta Catenin (Ser675) in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 catenin (cadherin-associated protein), beta 1, 88kDa
别名 Beta Catenin (Ser675), p Beta Catenin, p Beta Catenin (Ser675), p Beta Catenin Ser675, p-Beta Catenin
计算分子量 781 aa, 86 kDa
观测分子量 90-100 kDa
GenBank蛋白编号BC058926
基因名称 Beta Catenin
Gene ID (NCBI) 1499
ENSEMBL Gene IDENSG00000168036
RRIDAB_3673028
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP35222
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222). PKA was shown to phosphorylate β-catenin at Ser675. Phosphorylation at Ser675 induces β-catenin accumulation in the nucleus and increases its transcriptional activity.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Phospho-Beta Catenin (Ser675) antibody CL488-80084Download protocol
Standard Protocols
Click here to view our Standard Protocols