• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • FC experiment of A431 using 84839-1-RR (same clone as 84839-1-PBS)

    FC experiment of A431 using 84839-1-RR (same clone as 84839-1-PBS)

    1x10^6 EGF treated A431 cells were intracellularly stained with 0.25 ug Phospho-EGFR (Tyr869) Recombinant Antibody (84839-1-RR, Clone:241785G5) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 84839-1-PBS in a different storage buffer formulation.

  • WB analysis using 84839-1-RR (same clone as 84839-1-PBS)

    WB analysis using 84839-1-RR (same clone as 84839-1-PBS)

    Non-treated and EGF treated A431 cells were subjected to SDS PAGE followed by western blot with 84839-1-RR (Phospho-EGFR (Tyr869) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (66095-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 84839-1-PBS in a different storage buffer formulation.

Phospho-EGFR (Tyr869) Recombinant antibody, PBS Only

Phospho-EGFR (Tyr869) Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 84839-1-PBS

产品说明书

CloneNo. 241785G5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), Indirect ELISA

别名

EGFR, Proto-oncogene c-ErbB-1, Epidermal growth factor receptor, EGF Receptor , EGF R

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy EGFR Ready-To-Use IHC Kit. EGFR primary antibody included.

产品信息

84839-1-PBS targets Phospho-EGFR (Tyr869) in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
别名 EGFR, Proto-oncogene c-ErbB-1, Epidermal growth factor receptor, EGF Receptor , EGF R
计算分子量 1210 aa, 134 kDa
观测分子量180 kDa
GenBank蛋白编号BC094761
基因名称 EGFR
Gene ID (NCBI) 1956
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP00533
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Epidermal growth factor receptor (EGFR) is a member of the receptor tyrosine kinase (RTK) family and plays a critical role in a wide variety of cellular functions, including proliferation, differentiation and apoptosis. After EGFR homodimerization with extracellular ligands such as epidermal growth factor (EGF), the intracellular tyrosine kinase domain is activated and autophosphorylates multiple tyrosine residues in the C‐terminal tail domain. (PMID: 23822636) This antibody recognizes EGFR phosphorylation at tyrosine 869, which is also widely described as the tyrosine 845 site.