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验证数据展示

  • FC experiment of A431 using 84839-1-RR

    FC experiment of A431 using 84839-1-RR

    1x10^6 EGF treated A431 cells were intracellularly stained with 0.25 ug Phospho-EGFR (Tyr869) Recombinant Antibody (84839-1-RR, Clone:241785G5) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

  • WB analysis using 84839-1-RR

    WB analysis using 84839-1-RR

    Non-treated and EGF treated A431 cells were subjected to SDS PAGE followed by western blot with 84839-1-RR (Phospho-EGFR (Tyr869) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (66095-1-Ig) antibody as a loading control.

Phospho-EGFR (Tyr869) Recombinant antibody

Phospho-EGFR (Tyr869) Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84839-1-RR

Print datasheet

CloneNo. 241785G5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

EGFR Tyr845, EGFR (Tyr845), p-EGFR, p-EGFR (Tyr845), p-EGFR Tyr845

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inEGF treated A431 cells
Positive FC (Intra) detected inEGF treated A431 cells
Planning an IHC experiment? We recommend our IHCeasy EGFR Ready-To-Use IHC Kit. EGFR primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84839-1-RR targets Phospho-EGFR (Tyr869) in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
Synonyms EGFR Tyr845, EGFR (Tyr845), p-EGFR, p-EGFR (Tyr845), p-EGFR Tyr845
Calculated Molecular Weight 1210 aa, 134 kDa
Observed Molecular Weight180 kDa
GenBank Accession NumberBC094761
Gene Symbol EGFR
Gene ID (NCBI) 1956
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP00533
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Epidermal growth factor receptor (EGFR) is a member of the receptor tyrosine kinase (RTK) family and plays a critical role in a wide variety of cellular functions, including proliferation, differentiation and apoptosis. After EGFR homodimerization with extracellular ligands such as epidermal growth factor (EGF), the intracellular tyrosine kinase domain is activated and autophosphorylates multiple tyrosine residues in the C‐terminal tail domain. (PMID: 23822636) This antibody recognizes EGFR phosphorylation at tyrosine 869, which is also widely described as the tyrosine 845 site.

实验方案

Product Specific Protocols
WB protocol for Phospho-EGFR (Tyr869) antibody 84839-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols