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验证数据展示

  • WB analysis of HEK-293T using 84981-1-RR

    WB analysis of HEK-293T using 84981-1-RR

    Non-treated and Calyculin A treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 84981-1-RR (Phospho-EIF4E (Ser209) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

  • FC experiment of HEK-293T using 84981-1-RR

    FC experiment of HEK-293T using 84981-1-RR

    1x10^6 Calyculin A treated HEK-293T cells were intracellularly stained with 0.13 ug Phospho-EIF4E (Ser209) Recombinant antibody (84981-1-RR, Clone:242049G10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Phospho-EIF4E (Ser209) Recombinant antibody

Phospho-EIF4E (Ser209) Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84981-1-RR

产品说明书

CloneNo. 242049G10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

EIF4E (Ser209), p-EIF4E, p-EIF4E (Ser209), Phospho-EIF4E, Phospho-EIF4E Ser209

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCalyculin A treated HEK-293T cells
Positive FC (Intra) detected inCalyculin A treated HEK-293T cells
Planning an IHC experiment? We recommend our IHCeasy EIF4E Ready-To-Use IHC Kit. EIF4E primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84981-1-RR targets Phospho-EIF4E (Ser209) in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 eukaryotic translation initiation factor 4E
别名 EIF4E (Ser209), p-EIF4E, p-EIF4E (Ser209), Phospho-EIF4E, Phospho-EIF4E Ser209
计算分子量 29 kDa
观测分子量25 kDa
GenBank蛋白编号BC012611
基因名称 EIF4E
Gene ID (NCBI) 1977
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP06730
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Eukaryotic initiation factor 4F (eIF4F) is a critical effector and is thought to be the rate-limiting determinant of translation. The activity of eIF4E can be regulated by phosphorylation at Ser209, which is controlled by mitogen-activated protein kinase-interacting kinases Mnk1 and Mnk2. Although the function of eIF4E phosphorylation remains the subject of debate, it has been documented that the phosphorylation of eIF4E at Ser209 is crucial for biogenesis, tumorigenesis, and viral infection. (PMID: 38299843)

实验方案

Product Specific Protocols
WB protocol for Phospho-EIF4E (Ser209) antibody 84981-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols