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验证数据展示

  • WB analysis of HEK-293T using 84981-1-RR (same clone as 84981-1-PBS)

    WB analysis of HEK-293T using 84981-1-RR (same clone as 84981-1-PBS)

    Non-treated and Calyculin A treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 84981-1-RR (Phospho-EIF4E (Ser209) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 84981-1-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293T using 84981-1-RR (same clone as 84981-1-PBS)

    FC experiment of HEK-293T using 84981-1-RR (same clone as 84981-1-PBS)

    1x10^6 Calyculin A treated HEK-293T cells were intracellularly stained with 0.13 ug Phospho-EIF4E (Ser209) Recombinant antibody (84981-1-RR, Clone:242049G10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 84981-1-PBS in a different storage buffer formulation.

Phospho-EIF4E (Ser209) Recombinant antibody, PBS Only

Phospho-EIF4E (Ser209) Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 84981-1-PBS

产品说明书

CloneNo. 242049G10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), Indirect ELISA

别名

EIF4E, EIF4F, EIF4EL1, eIF-4E, eIF 4E

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy EIF4E Ready-To-Use IHC Kit. EIF4E primary antibody included.

产品信息

84981-1-PBS targets Phospho-EIF4E (Ser209) in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 eukaryotic translation initiation factor 4E
别名 EIF4E, EIF4F, EIF4EL1, eIF-4E, eIF 4E
计算分子量 29 kDa
观测分子量25 kDa
GenBank蛋白编号BC012611
基因名称 EIF4E
Gene ID (NCBI) 1977
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP06730
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Eukaryotic initiation factor 4F (eIF4F) is a critical effector and is thought to be the rate-limiting determinant of translation. The activity of eIF4E can be regulated by phosphorylation at Ser209, which is controlled by mitogen-activated protein kinase-interacting kinases Mnk1 and Mnk2. Although the function of eIF4E phosphorylation remains the subject of debate, it has been documented that the phosphorylation of eIF4E at Ser209 is crucial for biogenesis, tumorigenesis, and viral infection. (PMID: 38299843)