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Phospho-ERK1/2 (Thr202/Tyr204) Recombinant antibody, PBS Only

Phospho-ERK1/2 (Thr202/Tyr204) Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

8D12

Cat no : 80031-1-PBS

Print datasheet

Synonyms

ERK 1, ERK1, ERK1/2, ERT2, HS44KDAP, HUMKER1A, Insulin stimulated MAP2 kinase, MAP kinase 1, MAP kinase 3, MAP kinase isoform p44, MAPK 1, MAPK 3, MAPK3, p44 ERK1, p44 MAPK, P44ERK1, P44MAPK, Phospho-ERK1/2 (Thr202/Tyr204), PRKM3

验证数据展示

  • WB analysis using 80031-1-RR (same clone as 80031-1-PBS)

    WB analysis using 80031-1-RR (same clone as 80031-1-PBS)

    Non-treated HEK-293T and Calyculin A treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 80031-1-RR (Phospho-ERK1/2 (Thr202/Tyr204) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80031-1-PBS in a different storage buffer formulation.

  • WB analysis using 80031-1-RR (same clone as 80031-1-PBS)

    WB analysis using 80031-1-RR (same clone as 80031-1-PBS)

    Non-treated HeLa and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 80031-1-RR (Phospho-ERK1/2 (Thr202/Tyr204) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80031-1-PBS in a different storage buffer formulation.

产品信息

80031-1-PBS targets Phospho-ERK1/2 (Thr202/Tyr204) in WB, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name mitogen-activated protein kinase 3
Synonyms ERK 1, ERK1, ERK1/2, ERT2, HS44KDAP, HUMKER1A, Insulin stimulated MAP2 kinase, MAP kinase 1, MAP kinase 3, MAP kinase isoform p44, MAPK 1, MAPK 3, MAPK3, p44 ERK1, p44 MAPK, P44ERK1, P44MAPK, Phospho-ERK1/2 (Thr202/Tyr204), PRKM3
Calculated Molecular Weight 38-43 kDa
Observed Molecular Weight38-40 kDa
GenBank Accession NumberNM_002746
Gene Symbol ERK1
Gene ID (NCBI) 5595
RRIDAB_2918858
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP27361
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK and the transcription factor Elk-1.