验证数据展示
产品信息
66863-1-PBS targets Phospho-Histone H3 (Ser10) as part of a matched antibody pair:
MP50178-1: 68345-1-PBS capture and 66863-1-PBS detection (validated in Cytometric bead array)
Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
经测试应用 | WB, IHC, IF/ICC, IF-P, FC (Intra), Cytometric bead array, Indirect ELISA Application Description |
经测试反应性 | human, mouse, rat, pig |
免疫原 | Peptide 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | histone cluster 1, H3a |
别名 | Histone H3 (Ser10), p Histone H3, p Histone H3 (Ser10), p Histone H3 Ser10, p-Histone H3 |
计算分子量 | 15 kDa |
观测分子量 | 15-17 kDa |
GenBank蛋白编号 | NM_003529 |
基因名称 | HIST1H3A |
Gene ID (NCBI) | 8350 |
RRID | AB_2882201 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P68431 |
储存缓冲液 | PBS only , pH 7.3 |
储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
Phospho-histone-H3 (PHH3) is a core histone protein, which in its phosphorylated state forms the principal constituents of eukaryotic chromatin, with histone H3 being phosphorylated at serine (Ser) 10 or Ser28 as well as its phosphorylation of Ser10 being strongly correlated with the late G2 to M-phase transition in mammalian mitotic cells. On the basis of previous research, a few cell line- and animal model-based researches have displayed an increase in phosphorylation of histone H3 at Ser10 (H3S10ph), the only histone marker that is involved in carcinogenesis and cellular transformation. Histone H3 phosphorylation on serine-10 is specific to mitosis and phosphorylated histone H3 (PHH3) proliferation markers (as counts defined per area or as indices defined per cell numbers) are increasingly being used to evaluate proliferation in various tumors.