WB Figures
WB analysis using 66863-1-Ig (same clone as 66863-1-PBS)
Non-treated and Calyculin A treated cells were subjected to SDS PAGE followed by western blot with 66863-1-Ig (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66863-1-PBS in a different storage buffer formulation.
WB analysis using 66863-1-Ig (same clone as 66863-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66863-1-Ig (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66863-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 66863-1-Ig (same clone as 66863-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66863-1-Ig (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66863-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 66863-1-Ig (same clone as 66863-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66863-1-Ig (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66863-1-PBS in a different storage buffer formulation.
IHC staining of human renal cell carcinoma using 66863-1-Ig (same clone as 66863-1-PBS)
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66863-1-Ig (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66863-1-PBS in a different storage buffer formulation.
IHC staining of human renal cell carcinoma using 66863-1-Ig (same clone as 66863-1-PBS)
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66863-1-Ig (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66863-1-PBS in a different storage buffer formulation.
IHC staining of Jurkat using 66863-1-Ig (same clone as 66863-1-PBS)
Immunohistochemical analysis of paraffin-embedded Jurkat cells slide using 66863-1-Ig (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66863-1-PBS in a different storage buffer formulation.
IHC staining of Jurkat using 66863-1-Ig (same clone as 66863-1-PBS)
Immunohistochemical analysis of paraffin-embedded Jurkat cells slide using 66863-1-Ig (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66863-1-PBS in a different storage buffer formulation.
IHC staining of Jurkat using 66863-1-Ig (same clone as 66863-1-PBS)
Immunohistochemical analysis of paraffin-embedded Jurkat cells slide using 66863-1-Ig (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66863-1-PBS in a different storage buffer formulation.
IHC staining of Jurkat using 66863-1-Ig (same clone as 66863-1-PBS)
Immunohistochemical analysis of paraffin-embedded Jurkat cells slide using 66863-1-Ig (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66863-1-PBS in a different storage buffer formulation.
IHC staining of mouse heart using 66863-1-Ig (same clone as 66863-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66863-1-Ig (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66863-1-PBS in a different storage buffer formulation.
IHC staining of mouse kidney using 66863-1-Ig (same clone as 66863-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66863-1-Ig (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66863-1-PBS in a different storage buffer formulation.
IHC staining of mouse kidney using 66863-1-Ig (same clone as 66863-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66863-1-Ig (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66863-1-PBS in a different storage buffer formulation.
IHC staining of rat kidney using 66863-1-Ig (same clone as 66863-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 66863-1-Ig (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66863-1-PBS in a different storage buffer formulation.
IHC staining of rat kidney using 66863-1-Ig (same clone as 66863-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 66863-1-Ig (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66863-1-PBS in a different storage buffer formulation.
IF Figures
IF Staining of A549 using 66863-1-Ig (same clone as 66863-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed A549 cells using Phospho-Histone H3 (Ser10) antibody (66863-1-Ig, Clone: 4C7G2 ) at dilution of 1:1500 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, green). This data was developed using the same antibody clone with 66863-1-PBS in a different storage buffer formulation.
IF Staining of C2C12 using 66863-1-Ig (same clone as 66863-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using Phospho-Histone H3 (Ser10) antibody (66863-1-Ig, Clone: 4C7G2 ) at dilution of 1:1200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, green). This data was developed using the same antibody clone with 66863-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 66863-1-Ig (same clone as 66863-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Phospho-Histone H3 (Ser10) antibody (66863-1-Ig, Clone: 4C7G2 ) at dilution of 1:1500 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, green). This data was developed using the same antibody clone with 66863-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 66863-1-Ig (same clone as 66863-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Phospho-Histone H3 (Ser10) antibody (66863-1-Ig, Clone: 4C7G2 ) at dilution of 1:1500 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, green). This data was developed using the same antibody clone with 66863-1-PBS in a different storage buffer formulation.
IF Staining of human breast cancer using 66863-1-Ig (same clone as 66863-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 66863-1-Ig (PHH3 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66863-1-PBS in a different storage buffer formulation.
IF Staining of MCF-7 using 66863-1-Ig (same clone as 66863-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Phospho-Histone H3 (Ser10) antibody (66863-1-Ig, Clone: 4C7G2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66863-1-PBS in a different storage buffer formulation.
IF Staining of MCF-7 using 66863-1-Ig (same clone as 66863-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Phospho-Histone H3 (Ser10) antibody (66863-1-Ig, Clone: 4C7G2 ) at dilution of 1:1500 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, green). This data was developed using the same antibody clone with 66863-1-PBS in a different storage buffer formulation.
IF Staining of mouse testis using 66863-1-Ig (same clone as 66863-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using Phospho-Histone H3 (Ser10) antibody (66863-1-Ig, Clone: 4C7G2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66863-1-PBS in a different storage buffer formulation.
IF Staining of SKOV-3 using 66863-1-Ig (same clone as 66863-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using Phospho-Histone H3 (Ser10) antibody (66863-1-Ig, Clone: 4C7G2 ) at dilution of 1:1500 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, green). This data was developed using the same antibody clone with 66863-1-PBS in a different storage buffer formulation.
FC Figures
FC experiment of HepG2 using 66863-1-Ig (same clone as 66863-1-PBS)
1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human Phospho-Histone H3 (Ser10) (66863-1-Ig, Clone:4C7G2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.5 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (black). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100. This data was developed using the same antibody clone with 66863-1-PBS in a different storage buffer formulation.
FC experiment of HeLa using 66863-1-Ig (same clone as 66863-1-PBS)
1X10^6 nocodazole treated HeLa cells were intracellularly stained with 0.2 ug Anti-Human Phospho-Histone H3 (Ser10) (66863-1-Ig, Clone:4C7G2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 66863-1-PBS in a different storage buffer formulation.
FC experiment of HeLa using 66863-1-Ig (same clone as 66863-1-PBS)
1X10^6 nocodazole treated HeLa cells were intracellularly stained with 0.25 ug Anti-Human Phospho-Histone H3 (Ser10) (66863-1-Ig, Clone:4C7G2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.25 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 66863-1-PBS in a different storage buffer formulation.
CYTOMETRIC BEAD ARRAY Figures
Cytometric bead array in cell lysate using MP50178-1
Cytometric bead array in cell lysate using MP50178-1, Phospho-Histone H3 (Ser10) Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68345-1-PBS. Detection antibody: 66863-1-PBS. Cell lysate: Non-treated HeLa and Calyculin A treated HeLa (30μg/well). Non-related target OAT Monoclonal Matched Antibody Pair (MP50109-1P) was served as control.
