验证数据展示

WB analysis using 66863-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 66863-1-Ig (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
IF Staining of C2C12 using 66863-1-Ig
Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using Phospho-Histone H3 (Ser10) antibody (66863-1-Ig, Clone: 4C7G2 ) at dilution of 1:1200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, green).
IF Staining of HeLa using 66863-1-Ig
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Phospho-Histone H3 (Ser10) antibody (66863-1-Ig, Clone: 4C7G2 ) at dilution of 1:1500 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, green).
WB analysis using 66863-1-Ig
Non-treated and Calyculin A treated cells were subjected to SDS PAGE followed by western blot with 66863-1-Ig (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
IHC staining of Jurkat using 66863-1-Ig
Immunohistochemical analysis of paraffin-embedded Jurkat cells slide using 66863-1-Ig (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with Phospho-Histone H3 (Ser10) antibody included
IHC staining of Jurkat using 66863-1-Ig
Immunohistochemical analysis of paraffin-embedded Jurkat cells slide using 66863-1-Ig (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with Phospho-Histone H3 (Ser10) antibody included
IHC staining of Jurkat using 66863-1-Ig
Immunohistochemical analysis of paraffin-embedded Jurkat cells slide using 66863-1-Ig (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with Phospho-Histone H3 (Ser10) antibody included
IHC staining of Jurkat using 66863-1-Ig
Immunohistochemical analysis of paraffin-embedded Jurkat cells slide using 66863-1-Ig (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with Phospho-Histone H3 (Ser10) antibody included
IHC staining of human renal cell carcinoma using 66863-1-Ig
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66863-1-Ig (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with Phospho-Histone H3 (Ser10) antibody included
IHC staining of human renal cell carcinoma using 66863-1-Ig
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66863-1-Ig (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with Phospho-Histone H3 (Ser10) antibody included
IHC staining of mouse kidney using 66863-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66863-1-Ig (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with Phospho-Histone H3 (Ser10) antibody included
IHC staining of mouse kidney using 66863-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66863-1-Ig (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with Phospho-Histone H3 (Ser10) antibody included
IHC staining of rat kidney using 66863-1-Ig
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 66863-1-Ig (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with Phospho-Histone H3 (Ser10) antibody included
IHC staining of rat kidney using 66863-1-Ig
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 66863-1-Ig (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with Phospho-Histone H3 (Ser10) antibody included
IHC staining of human lung cancer using 66863-1-Ig
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66863-1-Ig (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with Phospho-Histone H3 (Ser10) antibody included
IHC staining of human lung cancer using 66863-1-Ig
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66863-1-Ig (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with Phospho-Histone H3 (Ser10) antibody included
IF Staining of mouse testis using 66863-1-Ig
Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using Phospho-Histone H3 (Ser10) antibody (66863-1-Ig, Clone: 4C7G2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
IF Staining of mouse testis using 66863-1-Ig
Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using Phospho-Histone H3 (Ser10) antibody (66863-1-Ig, Clone: 4C7G2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
IF Staining of mouse kidney using 66863-1-Ig
Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using Phospho-Histone H3 (Ser10) antibody (66863-1-Ig, Clone: 4C7G2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
IF Staining of human breast cancer using 66863-1-Ig
Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 66863-1-Ig (PHH3 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
IF Staining of MCF-7 using 66863-1-Ig
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Phospho-Histone H3 (Ser10) antibody (66863-1-Ig, Clone: 4C7G2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
IF Staining of HeLa using 66863-1-Ig
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Phospho-Histone H3 (Ser10) antibody (66863-1-Ig, Clone: 4C7G2 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), Beta Tubulin antibody (80713-1-RR, Clone: 2O13, red).
IF Staining of HeLa using 66863-1-Ig
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Phospho-Histone H3 (Ser10) antibody (66863-1-Ig, Clone: 4C7G2 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), Beta Tubulin antibody (80713-1-RR, Clone: 2O13, red).
IF Staining of HeLa using 66863-1-Ig
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Phospho-Histone H3 (Ser10) antibody (66863-1-Ig, Clone: 4C7G2 ) at dilution of 1:1500 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, green).
IF Staining of A549 using 66863-1-Ig
Immunofluorescent analysis of (4% PFA) fixed A549 cells using Phospho-Histone H3 (Ser10) antibody (66863-1-Ig, Clone: 4C7G2 ) at dilution of 1:1500 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, green).
IF Staining of MCF-7 using 66863-1-Ig
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Phospho-Histone H3 (Ser10) antibody (66863-1-Ig, Clone: 4C7G2 ) at dilution of 1:1500 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, green).
IF Staining of SKOV-3 using 66863-1-Ig
Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using Phospho-Histone H3 (Ser10) antibody (66863-1-Ig, Clone: 4C7G2 ) at dilution of 1:1500 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, green).
FC experiment of HeLa using 66863-1-Ig
1x10^6 nocodazole treated HeLa cells were intracellularly stained with 0.25 ug Phospho-Histone H3 (Ser10) Monoclonal antibody (66863-1-Ig, Clone:4C7G2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), and 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.
IHC staining of human breast cancer using 66863-1-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66863-1-Ig (H3S10-phospho antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with Phospho-Histone H3 (Ser10) antibody included

Phospho-Histone H3 (Ser10) Monoclonal antibody

Phospho-Histone H3 (Ser10) Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Cat No. 66863-1-Ig

Print datasheet

CloneNo. 4C7G2

Publications(17)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig and More (1)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA and More (1)

Synonyms

Histone H3 (Ser10), p Histone H3, p Histone H3 (Ser10), p Histone H3 Ser10, p-Histone H3

Formulation: PBS and Azide

PBS and Azide

PBS Only

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 647

Size:

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概述

经过测试的应用
产品信息
发表文章 (17)
实验方案

Print datasheet

MSDS

经过测试的应用

Positive WB detected in	HeLa cells, HEK-293 cells, Jurkat cells
Positive IHC detected in	human lung cancer tissue, human breast cancer tissue, human renal cell carcinoma tissue, Jurkat cells, mouse heart tissue, mouse kidney tissue, rat kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	human breast cancer tissue, mouse testis tissue, mouse kidney tissue
Positive IF/ICC detected in	C2C12 cells, MCF-7 cells, HeLa cells, A549 cells, SKOV-3 cells
Positive FC (Intra) detected in	nocodazole treated HeLa cells

Planning an IHC experiment? We recommend our IHCeasy Phospho-Histone H3 (Ser10) Ready-To-Use IHC Kit. Phospho-Histone H3 (Ser10) primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:1000-1:4000
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:600-1:2400
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WB	See 8 publications below
IHC	See 3 publications below
IF	See 7 publications below

产品信息

66863-1-Ig targets Phospho-Histone H3 (Ser10) in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications	WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA Application Description
Cited Applications	WB, IHC, IF
Tested Reactivity	human, mouse, rat, pig
Cited Reactivity	human, mouse, rat, chicken
Immunogen	Peptide 种属同源性预测
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Full Name	histone cluster 1, H3a
Synonyms	Histone H3 (Ser10), p Histone H3, p Histone H3 (Ser10), p Histone H3 Ser10, p-Histone H3
Calculated Molecular Weight	15 kDa
Observed Molecular Weight	15-17 kDa
GenBank Accession Number	NM_003529
Gene Symbol	HIST1H3A
Gene ID (NCBI)	8350
RRID	AB_2882201
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P68431
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

Phospho-histone-H3 (PHH3) is a core histone protein, which in its phosphorylated state forms the principal constituents of eukaryotic chromatin, with histone H3 being phosphorylated at serine (Ser) 10 or Ser28 as well as its phosphorylation of Ser10 being strongly correlated with the late G2 to M-phase transition in mammalian mitotic cells. On the basis of previous research, a few cell line- and animal model-based researches have displayed an increase in phosphorylation of histone H3 at Ser10 (H3S10ph), the only histone marker that is involved in carcinogenesis and cellular transformation. Histone H3 phosphorylation on serine-10 is specific to mitosis and phosphorylated histone H3 (PHH3) proliferation markers (as counts defined per area or as indices defined per cell numbers) are increasingly being used to evaluate proliferation in various tumors.

实验方案

Product Specific Protocols
WB protocol for Phospho-Histone H3 (Ser10) antibody 66863-1-Ig	Download protocol
IHC protocol for Phospho-Histone H3 (Ser10) antibody 66863-1-Ig	Download protocol
IF protocol for Phospho-Histone H3 (Ser10) antibody 66863-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	IHC	Oncogene Oncogenic long intervening noncoding RNA Linc00284 promotes c-Met expression by sponging miR-27a in colorectal cancer. Authors - Jun You View Article
human	WB	Biochem Pharmacol A novel aromatic amide derivative SY-65 co-targeted tubulin and histone deacetylase 1 with potent anticancer activity in vitro and in vivo. Authors - Yin-Ru Li View Article
human	IF	Front Mol Biosci High-Resolution Imaging of Tumor Spheroids and Organoids Enabled by Expansion Microscopy. Authors - Steven J Edwards View Article
mouse	IF	Sci Rep NF90-NF45 is essential for β cell compensation under obesity-inducing metabolic stress through suppression of p53 signaling pathway. Authors - Sylvia Lai View Article
chicken	IHC	Dev Dyn The dynamic spatial and temporal relationships between the phalanx-forming region and the interdigits determine digit identity in the chick limb autopod. Authors - Takayuki Suzuki View Article
	WB	bioRxiv Palmitoylated Importin α Regulates Mitotic Spindle Orientation Through Interaction with NuMA Authors - Patrick James Sutton View Article

验证数据展示

WB analysis using 66863-1-Ig

IF Staining of C2C12 using 66863-1-Ig

IF Staining of HeLa using 66863-1-Ig

WB analysis using 66863-1-Ig

IHC staining of Jurkat using 66863-1-Ig

IHC staining of Jurkat using 66863-1-Ig

IHC staining of Jurkat using 66863-1-Ig

IHC staining of Jurkat using 66863-1-Ig

IHC staining of human renal cell carcinoma using 66863-1-Ig

IHC staining of human renal cell carcinoma using 66863-1-Ig

IHC staining of mouse kidney using 66863-1-Ig

IHC staining of mouse kidney using 66863-1-Ig

IHC staining of rat kidney using 66863-1-Ig

IHC staining of rat kidney using 66863-1-Ig

IHC staining of human lung cancer using 66863-1-Ig

IHC staining of human lung cancer using 66863-1-Ig

IF Staining of mouse testis using 66863-1-Ig

IF Staining of mouse testis using 66863-1-Ig

IF Staining of mouse kidney using 66863-1-Ig

IF Staining of human breast cancer using 66863-1-Ig

IF Staining of MCF-7 using 66863-1-Ig

IF Staining of HeLa using 66863-1-Ig

IF Staining of HeLa using 66863-1-Ig

IF Staining of HeLa using 66863-1-Ig

IF Staining of A549 using 66863-1-Ig

IF Staining of MCF-7 using 66863-1-Ig

IF Staining of SKOV-3 using 66863-1-Ig

FC experiment of HeLa using 66863-1-Ig

IHC staining of human breast cancer using 66863-1-Ig

Phospho-Histone H3 (Ser10) Monoclonal antibody

Phospho-Histone H3 (Ser10) Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

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经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Oncogenic long intervening noncoding RNA Linc00284 promotes c-Met expression by sponging miR-27a in colorectal cancer.

A novel aromatic amide derivative SY-65 co-targeted tubulin and histone deacetylase 1 with potent anticancer activity in vitro and in vivo.

High-Resolution Imaging of Tumor Spheroids and Organoids Enabled by Expansion Microscopy.

NF90-NF45 is essential for β cell compensation under obesity-inducing metabolic stress through suppression of p53 signaling pathway.

The dynamic spatial and temporal relationships between the phalanx-forming region and the interdigits determine digit identity in the chick limb autopod.

Palmitoylated Importin α Regulates Mitotic Spindle Orientation Through Interaction with NuMA