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验证数据展示

  • FC experiment of HeLa using CL488-66863

    FC experiment of HeLa using CL488-66863

    1X10^6 HeLa cells untreated(dashed lines) or treated with nocodazole (red) were intracellularly stained with 0.5 ug CoraLite® Plus 488 Anti-Human Phospho-Histone H3 (Ser10) (CL488-66863, Clone:4C7G2), or 0.5 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

CoraLite® Plus 488-conjugated Phospho-Histone H3 (Ser10) Monoclonal antibody

Phospho-Histone H3 (Ser10) Monoclonal Antibody for FC (Intra)
Cat No. CL488-66863

产品说明书

CloneNo. 4C7G2

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

FC (Intra)

别名

HIST1H3A, 4C7G2, Histone-H3, HistoneH3, Histone H3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected innocodazole treated HeLa cells
Planning an IHC experiment? We recommend our IHCeasy Phospho-Histone H3 (Ser10) Ready-To-Use IHC Kit. Phospho-Histone H3 (Ser10) primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66863-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66863 targets Phospho-Histone H3 (Ser10) in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 histone cluster 1, H3a
别名 HIST1H3A, 4C7G2, Histone-H3, HistoneH3, Histone H3
计算分子量 15 kDa
观测分子量 15-17 kDa
GenBank蛋白编号NM_003529
基因名称 HIST1H3A
Gene ID (NCBI) 8350
RRIDAB_2923760
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Phospho-histone-H3 (PHH3) is a core histone protein, which in its phosphorylated state forms the principal constituents of eukaryotic chromatin, with histone H3 being phosphorylated at serine (Ser) 10 or Ser28 as well as its phosphorylation of Ser10 being strongly correlated with the late G2 to M-phase transition in mammalian mitotic cells. On the basis of previous research, a few cell line- and animal model-based researches have displayed an increase in phosphorylation of histone H3 at Ser10 (H3S10ph), the only histone marker that is involved in carcinogenesis and cellular transformation. Histone H3 phosphorylation on serine-10 is specific to mitosis and phosphorylated histone H3 (PHH3) proliferation markers (as counts defined per area or as indices defined per cell numbers) are increasingly being used to evaluate proliferation in various tumors.