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Phospho-IRF3 (Ser396) Recombinant antibody, PBS Only

Phospho-IRF3 (Ser396) Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241522A6

Cat no : 80519-2-PBS

Print datasheet

Synonyms

IRF3, IRF-3, IRF 3, interferon regulatory factor 3, 241522A6

验证数据展示

  • WB analysis of THP-1 using 80519-2-RR (same clone as 80519-2-PBS)

    WB analysis of THP-1 using 80519-2-RR (same clone as 80519-2-PBS)

    Phosphatase inhibitor treated or λ phosphatase treated THP-1 cells were subjected to SDS PAGE followed by western blot with 80519-2-RR (Phospho-IRF3 (Ser396) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 80519-2-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293T using 80519-2-RR (same clone as 80519-2-PBS)

    FC experiment of HEK-293T using 80519-2-RR (same clone as 80519-2-PBS)

    1x10^6 Calyculin A treated HEK-293T cells were intracellularly stained with 0.25 ug Phospho-IRF3 (Ser396) Recombinant antibody (80519-2-RR, Clone:241522A6) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 80519-2-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy IRF3 Ready-To-Use IHC Kit. IRF3 primary antibody included.

产品信息

80519-2-PBS targets Phospho-IRF3 (Ser396) in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interferon regulatory factor 3
Synonyms IRF3, IRF-3, IRF 3, interferon regulatory factor 3, 241522A6
Calculated Molecular Weight 47 kDa
Observed Molecular Weight55-65 kDa
GenBank Accession NumberBC009395
Gene Symbol IRF3
Gene ID (NCBI) 3661
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ14653
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The virul-induced expression of interferon(IFN) genes in infected cells implicate in the interplay of several constitutively expressed and virus-activated transcription factors. A family of IFN regulatory factors(IRFs) have been shown to has a role in the transcription of IFN genes as well as IFN-stimulated genes. IRF3 is a novel key transcriptional regulator of type I IFN-dependent immune responses and involves in the innate immune response against DNA and RNA viruses, by binding to the promoters of IFN. It located in the cytoplasm of uninfected cells in an inactive form, and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, could be phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes.