Phospho-IRF3 (Ser396) Recombinant antibody

Phospho-IRF3 (Ser396) Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 80519-2-RR

产品说明书

CloneNo. 241522A6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

IRF3 (Ser396), p IRF3, p IRF3 (Ser396), p IRF3 Ser396, Phospho IRF3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inλ phosphatase treated THP-1 cells
Positive FC (Intra) detected inCalyculin A treated HEK-293T cells
Planning an IHC experiment? We recommend our IHCeasy IRF3 Ready-To-Use IHC Kit. IRF3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80519-2-RR targets Phospho-IRF3 (Ser396) in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 interferon regulatory factor 3
别名 IRF3 (Ser396), p IRF3, p IRF3 (Ser396), p IRF3 Ser396, Phospho IRF3
计算分子量 47 kDa
观测分子量55-65 kDa
GenBank蛋白编号BC009395
基因名称 IRF3
Gene ID (NCBI) 3661
RRIDAB_3670478
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ14653
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The virul-induced expression of interferon(IFN) genes in infected cells implicate in the interplay of several constitutively expressed and virus-activated transcription factors. A family of IFN regulatory factors(IRFs) have been shown to has a role in the transcription of IFN genes as well as IFN-stimulated genes. IRF3 is a novel key transcriptional regulator of type I IFN-dependent immune responses and involves in the innate immune response against DNA and RNA viruses, by binding to the promoters of IFN. It located in the cytoplasm of uninfected cells in an inactive form, and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, could be phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes.

实验方案

Product Specific Protocols
WB protocol for Phospho-IRF3 (Ser396) antibody 80519-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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