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验证数据展示

  • WB analysis of THP-1 using 80519-2-RR

    WB analysis of THP-1 using 80519-2-RR

    Phosphatase inhibitor treated or λ phosphatase treated THP-1 cells were subjected to SDS PAGE followed by western blot with 80519-2-RR (Phospho-IRF3 (Ser396) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

  • FC experiment of HEK-293T using 80519-2-RR

    FC experiment of HEK-293T using 80519-2-RR

    1x10^6 Calyculin A treated HEK-293T cells were intracellularly stained with 0.25 ug Phospho-IRF3 (Ser396) Recombinant antibody (80519-2-RR, Clone:241522A6) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Phospho-IRF3 (Ser396) Recombinant antibody

Phospho-IRF3 (Ser396) Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 80519-2-RR

Print datasheet

CloneNo. 241522A6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

IRF3 (Ser396), p IRF3, p IRF3 (Ser396), p IRF3 Ser396, Phospho IRF3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inλ phosphatase treated THP-1 cells
Positive FC (Intra) detected inCalyculin A treated HEK-293T cells
Planning an IHC experiment? We recommend our IHCeasy IRF3 Ready-To-Use IHC Kit. IRF3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80519-2-RR targets Phospho-IRF3 (Ser396) in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interferon regulatory factor 3
Synonyms IRF3 (Ser396), p IRF3, p IRF3 (Ser396), p IRF3 Ser396, Phospho IRF3
Calculated Molecular Weight 47 kDa
Observed Molecular Weight55-65 kDa
GenBank Accession NumberBC009395
Gene Symbol IRF3
Gene ID (NCBI) 3661
RRIDAB_3670478
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ14653
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The virul-induced expression of interferon(IFN) genes in infected cells implicate in the interplay of several constitutively expressed and virus-activated transcription factors. A family of IFN regulatory factors(IRFs) have been shown to has a role in the transcription of IFN genes as well as IFN-stimulated genes. IRF3 is a novel key transcriptional regulator of type I IFN-dependent immune responses and involves in the innate immune response against DNA and RNA viruses, by binding to the promoters of IFN. It located in the cytoplasm of uninfected cells in an inactive form, and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, could be phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes.

实验方案

Product Specific Protocols
WB protocol for Phospho-IRF3 (Ser396) antibody 80519-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols