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Phospho-IkB Alpha (Ser32/36) Recombinant antibody, PBS Only

Phospho-IkB Alpha (Ser32/36) Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G21

Cat no : 82349-1-PBS

Print datasheet

Synonyms

IKBA, IkB alpha, IκB α, NFKBIA, I-kappa-B-alpha

验证数据展示

  • WB analysis of HeLa using 82349-1-RR (same clone as 82349-1-PBS)

    WB analysis of HeLa using 82349-1-RR (same clone as 82349-1-PBS)

    Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 82349-1-RR (Phospho-IkB Alpha (Ser32/36) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as loading control. This data was developed using the same antibody clone with 82349-1-PBS in a different storage buffer formulation.

  • IHC staining of human skin cancer using 82349-1-RR (same clone as 82349-1-PBS)

    IHC staining of human skin cancer using 82349-1-RR (same clone as 82349-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 82349-1-RR (Phospho-IkB Alpha (Ser32/36) antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82349-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NFKBIA antibody included
  • IHC staining of human skin cancer using 82349-1-RR (same clone as 82349-1-PBS)

    IHC staining of human skin cancer using 82349-1-RR (same clone as 82349-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 82349-1-RR (Phospho-IkB Alpha (Ser32/36) antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82349-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NFKBIA antibody included
Planning an IHC experiment? We recommend our IHCeasy NFKBIA Ready-To-Use IHC Kit. NFKBIA primary antibody included.

产品信息

82349-1-PBS targets Phospho-IkB Alpha (Ser32/36) in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
Synonyms IKBA, IkB alpha, IκB α, NFKBIA, I-kappa-B-alpha
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC004983
Gene Symbol IkB Alpha
Gene ID (NCBI) 4792
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP25963
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime. The antibody also detects the phosphorylated form of the NFKBIA (IkB Alpha) (PMID: 16904979, PMID: 28990531 PMID: 10455908).