Phospho-IkB Alpha (Ser32/36) Recombinant antibody

Phospho-IkB Alpha (Ser32/36) Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 82349-1-RR

产品说明书

CloneNo. 1G21

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, FC (Intra), ELISA and More (1)

IκBα (Ser 32/36), p IkB Alpha, p IkB Alpha (Ser32/36), p IkB Alpha Ser32/36, p IkB α

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inCalyculin A treated HeLa cells, Calyculin A treated HSC-T6 cells, Calyculin A treated NIH/3T3 cells, rat liver tissue, mouse liver tissue
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inCalyculin A treated HeLa cells
Planning an IHC experiment? We recommend our IHCeasy NFKBIA Ready-To-Use IHC Kit. NFKBIA primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.06 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82349-1-RR targets Phospho-IkB Alpha (Ser32/36) in WB, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, pig
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
别名 IκBα (Ser 32/36), p IkB Alpha, p IkB Alpha (Ser32/36), p IkB Alpha Ser32/36, p IkB α
计算分子量 36 kDa
观测分子量 36 kDa
GenBank蛋白编号BC004983
基因名称 IkB Alpha
Gene ID (NCBI) 4792
RRIDAB_3073626
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP25963
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime. The antibody also detects the phosphorylated form of the NFKBIA (IkB Alpha) (PMID: 16904979, PMID: 28990531 PMID: 10455908).

实验方案

Product Specific Protocols
WB protocol for Phospho-IkB Alpha (Ser32/36) antibody 82349-1-RRDownload protocol
IHC protocol for Phospho-IkB Alpha (Ser32/36) antibody 82349-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB

Int J Biol Macromol

In vitro and in vivo immunomodulatory activity of acetylated polysaccharides from Cyclocarya paliurus leaves

Authors - Yang Zhang
mouseWB

Phytomedicine

Aucklandiae radix targeted PKM2 to alleviate ulcerative colitis: Insights from the photocrosslinking target fishing technique

Authors - Yan-Li Feng
humanWB

Sci Rep

LncRNA DDX11-AS1 promotes breast cancer progression by targeting the miR-30c-5p/MTDH axis

Authors - Yanting Li
mouseWB

J Cell Biochem

Dehydrocostus Lactone Ameliorates LPS-Induced Acute Lung Injury by Inhibiting PFKFB3-Mediated Glycolysis

Authors - Yue Li
mouseWB

Biol Trace Elem Res

BPA Exacerbates Zinc Deficiency-Induced Testicular Tissue Inflammation in Male Mice Through the TNF-α/NF-κB/Caspase8 Signaling Pathway

Authors - Xinying Wang
mouseWB

Front Pharmacol

Shikimic acid (SA) inhibits neuro-inflammation and exerts neuroprotective effects in an LPS-induced in vitro and in vivo model

Authors - Xueying Bao
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