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  • Featured Product
  • KD/KO Validated

Phospho-MEK1 (Thr386) Recombinant antibody, PBS Only

Phospho-MEK1 (Thr386) Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

6K5

Cat no : 81304-1-PBS

Print datasheet

Synonyms

MAP2K1, MEK1, MAPKK 1, MAP2K 1, EC:2.7.12.2

验证数据展示

  • WB analysis of HeLa using 81304-1-RR (same clone as 81304-1-PBS)

    WB analysis of HeLa using 81304-1-RR (same clone as 81304-1-PBS)

    Non-treated HeLa cells, phosphatase inhibitor treated and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 81304-1-RR (Phospho-MEK1 (Thr386) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 81304-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 81304-1-RR (same clone as 81304-1-PBS)

    WB analysis of HeLa using 81304-1-RR (same clone as 81304-1-PBS)

    WB result of Phospho-MEK1 (Thr386) antibody (81304-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Phospho-MEK1 (Thr386) transfected HeLa cells. This data was developed using the same antibody clone with 81304-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 81304-1-RR (same clone as 81304-1-PBS)

    FC experiment of HeLa using 81304-1-RR (same clone as 81304-1-PBS)

    1X10^6 HeLa cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.06 ug Phospho-MEK1 (Thr386) Recombinant antibody (81304-1-RR, Clone:6K5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 81304-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy MAP2K1/MEK1 Ready-To-Use IHC Kit. MAP2K1/MEK1 primary antibody included.

产品信息

81304-1-PBS targets Phospho-MEK1 (Thr386) in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name mitogen-activated protein kinase kinase 1
Synonyms MAP2K1, MEK1, MAPKK 1, MAP2K 1, EC:2.7.12.2
Calculated Molecular Weight 43 kDa
Observed Molecular Weight40-50 kDa
GenBank Accession NumberBC139729
Gene Symbol MEK1
Gene ID (NCBI) 5604
ENSEMBL Gene IDENSG00000169032
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ02750
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MAP2K1 encodes MAPK1, also known as MEK1. MEK1 variants can enhance MEK1 expression and ERK1 phosphorylation that together lead to continuous activation of MEK/ERK signaling pathway. MEK1 bind directly to ERK2 through a region in the N terminus of MEK. In addition, a proline-rich (PR) regulatory sequence in MEK is also involved in MEK-ERK association and signal propagation. The coupling between MEK1 and ERK2 is enhanced through phosphorylation on S298 in the MEK1 PR region, whereas phosphorylation on MEK1 T292 releases the complex. MEK1 T292 is a substrate of ERK2, but the site is also phosphorylated at a basal level when ERK2 is inhibited, suggesting several regulators of this site . Although the S298 site in MEK2 has been conserved, it lacks the T292 phosphorylation site, and it is not a substrate of PAK1. (PMID: 31972311, PMID: 17928366, PMID: 22177953)