• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

Phospho-MEK1 (Thr386) Recombinant antibody

Phospho-MEK1 (Thr386) Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

6K5

Cat no : 81304-1-RR

Print datasheet

Synonyms

MAP2K1, MEK1, MAPKK 1, MAP2K 1, EC:2.7.12.2

验证数据展示

  • WB analysis of HeLa using 81304-1-RR

    WB analysis of HeLa using 81304-1-RR

    Non-treated HeLa cells, phosphatase inhibitor treated and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 81304-1-RR (Phospho-MEK1 (Thr386) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

  • WB analysis of HeLa using 81304-1-RR

    WB analysis of HeLa using 81304-1-RR

    WB result of Phospho-MEK1 (Thr386) antibody (81304-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Phospho-MEK1 (Thr386) transfected HeLa cells.

  • FC experiment of HeLa using 81304-1-RR

    FC experiment of HeLa using 81304-1-RR

    1X10^6 HeLa cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.06 ug Phospho-MEK1 (Thr386) Recombinant antibody (81304-1-RR, Clone:6K5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

经过测试的应用

Positive WB detected inHeLa cells, λ phosphatase treated HeLa cells
Positive FC (Intra) detected inλ phosphatase treated HeLa cells
Planning an IHC experiment? We recommend our IHCeasy MAP2K1/MEK1 Ready-To-Use IHC Kit. MAP2K1/MEK1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.06 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

81304-1-RR targets Phospho-MEK1 (Thr386) in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name mitogen-activated protein kinase kinase 1
Synonyms MAP2K1, MEK1, MAPKK 1, MAP2K 1, EC:2.7.12.2
Calculated Molecular Weight 43 kDa
Observed Molecular Weight40-50 kDa
GenBank Accession NumberBC139729
Gene Symbol MEK1
Gene ID (NCBI) 5604
ENSEMBL Gene IDENSG00000169032
RRIDAB_2923714
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ02750
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAP2K1 encodes MAPK1, also known as MEK1. MEK1 variants can enhance MEK1 expression and ERK1 phosphorylation that together lead to continuous activation of MEK/ERK signaling pathway. MEK1 bind directly to ERK2 through a region in the N terminus of MEK. In addition, a proline-rich (PR) regulatory sequence in MEK is also involved in MEK-ERK association and signal propagation. The coupling between MEK1 and ERK2 is enhanced through phosphorylation on S298 in the MEK1 PR region, whereas phosphorylation on MEK1 T292 releases the complex. MEK1 T292 is a substrate of ERK2, but the site is also phosphorylated at a basal level when ERK2 is inhibited, suggesting several regulators of this site . Although the S298 site in MEK2 has been conserved, it lacks the T292 phosphorylation site, and it is not a substrate of PAK1. (PMID: 31972311, PMID: 17928366, PMID: 22177953)

实验方案

Product Specific Protocols
WB protocol for Phospho-MEK1 (Thr386) antibody 81304-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Biomed Pharmacother

An anti-RAGE chimeric antibody alleviates CCl4-induced liver fibrosis via RAGE/NF-kB pathway in mice

Authors - Jing Liu
View Article