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验证数据展示

  • WB analysis using 82717-1-RR

    WB analysis using 82717-1-RR

    Non-treated and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 82717-1-RR (Phospho-MYL9 (Thr19/Ser20) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin antibody as loading control.

  • FC experiment of HeLa using 82717-1-RR

    FC experiment of HeLa using 82717-1-RR

    1x10^6 Calyculin A treated HeLa cells were intracellularly stained with 0.25 ug Phospho-MYL9 (Thr19/Ser20) Recombinant antibody (82717-1-RR, Clone:1G10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Phospho-MYL9 (Thr19/Ser20) Recombinant antibody

Phospho-MYL9 (Thr19/Ser20) Recombinant Antibody for WB, ELISA
Cat No. 82717-1-RR

产品说明书

CloneNo. 1G10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

MYL9, MLC-2C, MLC2, 1G10

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCalyculin A treated NIH/3T3 cells, Calyculin A treated HeLa cells
Positive FC (Intra) detected inCalyculin A treated HeLa cells

This antibody, also known as Thr18/Ser19, is the site where the initiator methionine is removed.

Planning an IHC experiment? We recommend our IHCeasy MYL9 Ready-To-Use IHC Kit. MYL9 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82717-1-RR targets Phospho-MYL9 (Thr19/Ser20) in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 myosin, light chain 9, regulatory
别名 MYL9, MLC-2C, MLC2, 1G10
计算分子量 20 kDa
观测分子量19-20 kDa
GenBank蛋白编号BC002648
基因名称 MYL9
Gene ID (NCBI) 10398
RRIDAB_3085363
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP24844
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Myosin regulatory light polypeptide 9 (MYL9), also known as MLC2, belongs to the myosin regulatory subunits. It plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation at Thr19 and Ser20. Implicated in cytokinesis, receptor capping, and cell locomotion (PMID:11942626, PMID:2526655). Some studies have demonstrated that MYL9 may play important roles in various human cancers. The expression and phosphorylation of MYL9 (Thr19/Ser20) may be increased in human breast (PMID: 22144583) and liver cancers (PMID: 18648664), while decreased in human colon (PMID: 22752057) and bladder cancers (PMID: 21139803). MYL9 was the only gene differentially expressed in the aged versus young injured arteries in the rat smooth muscle cell layers (PMID:22003410).

实验方案

Product Specific Protocols
WB protocol for Phospho-MYL9 (Thr19/Ser20) antibody 82717-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols