Phospho-MYL9 (Thr19/Ser20) Recombinant antibody
Phospho-MYL9 (Thr19/Ser20) Uni-rAbTM Recombinant Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
1G10
验证数据展示
经过测试的应用
Positive WB detected in | Calyculin A treated NIH/3T3 cells, Calyculin A treated HeLa cells |
Positive FC (Intra) detected in | Calyculin A treated HeLa cells |
This antibody, also known as Thr18/Ser19, is the site where the initiator methionine is removed.
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
82717-1-RR targets Phospho-MYL9 (Thr19/Ser20) in WB, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, ELISA Application Description |
Tested Reactivity | human, mouse |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | myosin, light chain 9, regulatory |
Synonyms | MYL9, MLC-2C, MLC2, 1G10 |
Calculated Molecular Weight | 20 kDa |
Observed Molecular Weight | 19-20 kDa |
GenBank Accession Number | BC002648 |
Gene Symbol | MYL9 |
Gene ID (NCBI) | 10398 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P24844 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for Phospho-MYL9 (Thr19/Ser20) antibody 82717-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |