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Conjugate
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验证数据展示

  • WB analysis using 80195-1-RR (same clone as 80195-1-PBS)

    WB analysis using 80195-1-RR (same clone as 80195-1-PBS)

    Non-treated and etoposide treated HT-29 cells were subjected to SDS PAGE followed by western blot with 80195-1-RR (Phospho-P53 (Ser15) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 80195-1-PBS in a different storage buffer formulation.

  • WB analysis using 80195-1-RR (same clone as 80195-1-PBS)

    WB analysis using 80195-1-RR (same clone as 80195-1-PBS)

    Non-treated and UV treated A431 cells were subjected to SDS PAGE followed by western blot with 80195-1-RR (Phospho-P53 (Ser15) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 80195-1-PBS in a different storage buffer formulation.

  • IF Staining of HT-29 using 80195-1-RR (same clone as 80195-1-PBS)

    IF Staining of HT-29 using 80195-1-RR (same clone as 80195-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed etoposide treated HT-29 cells and non-treated HT-29 cells using Phospho-P53 (Ser15) antibody (80195-1-RR, Clone: 2J21 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80195-1-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 80195-1-RR (same clone as 80195-1-PBS)

    FC experiment of A431 using 80195-1-RR (same clone as 80195-1-PBS)

    1X10^6 A431 cells untreated (dashed line) or treated with UV (red) were intracellularly stained with 0.13 ug Anti-Human Phospho-P53 (Ser15) (80195-1-RR, Clone:2J21) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or 0.13 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 80195-1-PBS in a different storage buffer formulation.

Phospho-P53 (Ser15) Recombinant antibody, PBS Only

Phospho-P53 (Ser15) Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 80195-1-PBS

产品说明书

CloneNo. 2J21

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), Indirect ELISA

别名

TP53, P53, Tumor suppressor p53, tumor protein p53, Antigen NY-CO-13

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy P53 Ready-To-Use IHC Kit. P53 primary antibody included.

产品信息

80195-1-PBS targets Phospho-P53 (Ser15) in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tumor protein p53
别名 TP53, P53, Tumor suppressor p53, tumor protein p53, Antigen NY-CO-13
计算分子量 44 kDa
观测分子量 53 kDa
GenBank蛋白编号BC003596
基因名称 P53
Gene ID (NCBI) 7157
RRIDAB_2918873
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP04637
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

P53 is a 53 kDa protein that is activated in response to alteration of normal cell homeostasis, including DNA damage, nutrient starvation, heat shock, virus infection, pH change, hypoxia, and oncogene activation. P53 maintains genetic stability by regulating different processes, such as cell-cycle arrest, DNA synthesis and repair, programmed cell death, and energy metabolism. In non-stressed conditions these proteins bind p53, ubiquitylate it and target it for degradation by the proteasome. In stressed conditions the function of the MdM2-MdM4 complex is blocked by phosphorylation, protein-binding events and/or enhanced degradation. (PMID: 19935675, PMID: 24379683)