Phospho-P53 (Ser15) Recombinant antibody

Phospho-P53 (Ser15) Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 80195-1-RR

产品说明书

CloneNo. 2J21

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA

TP53, P53, Tumor suppressor p53, tumor protein p53, Antigen NY-CO-13

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
规格: 

-/ -


经过测试的应用

Positive WB detected inetoposide treated HT-29 cells, UV treated A431 cells
Positive IF/ICC detected inetoposide treated HT-29 cells
Positive FC (Intra) detected inUV treated A431 cells
Planning an IHC experiment? We recommend our IHCeasy P53 Ready-To-Use IHC Kit. P53 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

80195-1-RR targets Phospho-P53 (Ser15) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tumor protein p53
别名 TP53, P53, Tumor suppressor p53, tumor protein p53, Antigen NY-CO-13
计算分子量 44 kDa
观测分子量 53 kDa
GenBank蛋白编号BC003596
基因名称 P53
Gene ID (NCBI) 7157
RRIDAB_2918873
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP04637
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

P53 is a 53 kDa protein that is activated in response to alteration of normal cell homeostasis, including DNA damage, nutrient starvation, heat shock, virus infection, pH change, hypoxia, and oncogene activation. P53 maintains genetic stability by regulating different processes, such as cell-cycle arrest, DNA synthesis and repair, programmed cell death, and energy metabolism. In non-stressed conditions these proteins bind p53, ubiquitylate it and target it for degradation by the proteasome. In stressed conditions the function of the MdM2-MdM4 complex is blocked by phosphorylation, protein-binding events and/or enhanced degradation. (PMID: 19935675, PMID: 24379683)

实验方案

Product Specific Protocols
WB protocol for Phospho-P53 (Ser15) antibody 80195-1-RRDownload protocol
IF protocol for Phospho-P53 (Ser15) antibody 80195-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Sci Rep

Involvement of JNK signaling in Aspergillus fumigatus-induced inflammatory factors release in bronchial epithelial cells

Authors - Xiao Cui

Cells

Concurrent Activation of Both Survival-Promoting and Death-Inducing Signaling by Chloroquine in Glioblastoma Stem Cells: Implications for Potential Risks and Benefits of Using Chloroquine as Radiosensitizer

Authors - Andreas Müller
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