CoraLite® Plus 647-conjugated Phospho-P53 (Ser15) Recombinant antibody
Phospho-P53 (Ser15) Recombinant Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
IF/ICC
Conjugate
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
2J21
验证数据展示
经过测试的应用
Positive IF detected in | etoposide treated HT-29 cells |
Planning an IHC experiment? We recommend our IHCeasy P53 Ready-To-Use IHC Kit. P53 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 80195-1-PBS
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL647-80195 targets Phospho-P53 (Ser15) in IF applications and shows reactivity with Human samples.
Tested Applications | IF/ICC Application Description |
Tested Reactivity | Human |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | tumor protein p53 |
Synonyms | Antigen NY CO 13, Cellular tumor antigen p53, FLJ92943, LFS1, P53, Phosphoprotein p53, TP53, TRP53, tumor protein p53, Tumor suppressor p53 |
Calculated Molecular Weight | 44 kDa |
Observed Molecular Weight | 53 kDa |
GenBank Accession Number | BC003596 |
Gene Symbol | TP53 |
Gene ID (NCBI) | 7157 |
Conjugate | CoraLite® Plus 647 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P04637 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
P53 is a 53 kDa protein that is activated in response to alteration of normal cell homeostasis, including DNA damage, nutrient starvation, heat shock, virus infection, pH change, hypoxia, and oncogene activation. P53 maintains genetic stability by regulating different processes, such as cell-cycle arrest, DNA synthesis and repair, programmed cell death, and energy metabolism. In non-stressed conditions these proteins bind p53, ubiquitylate it and target it for degradation by the proteasome. In stressed conditions the function of the MdM2-MdM4 complex is blocked by phosphorylation, protein-binding events and/or enhanced degradation. (PMID: 19935675, PMID: 24379683)
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 647 Phospho-P53 (Ser15) antibody CL647-80195 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |