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CoraLite®594-conjugated Phospho-P53 (Ser15) Recombinant antibody

Phospho-P53 (Ser15) Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2J21

Cat no : CL594-80195

Print datasheet

Synonyms

TP53, P53, Tumor suppressor p53, tumor protein p53, Antigen NY-CO-13

验证数据展示

  • FC experiment of A431 using CL594-80195

    FC experiment of A431 using CL594-80195

    1X10^6 A431 cells untreated (dashed line) or UV treated A431 cells (red) were intracellularly stained with 0.5 ug CoraLite®594 Anti-Human Phospho-P53 (Ser15) (CL594-80195, Clone:2J21) , or 0.5 ug Control Antibody (Blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

  • IF Staining of HT-29 using CL594-80195

    IF Staining of HT-29 using CL594-80195

    Immunofluorescent analysis of (4% PFA) fixed etoposide treated HT-29 cells using CoraLite®594 Phospho-P53 (Ser15) antibody (CL594-80195, Clone: 2J21 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inetoposide treated HT-29 cells
Positive FC (Intra) detected inUV treated A431 cells
Planning an IHC experiment? We recommend our IHCeasy P53 Ready-To-Use IHC Kit. P53 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 80195-1-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-80195 targets Phospho-P53 (Ser15) in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name tumor protein p53
Synonyms TP53, P53, Tumor suppressor p53, tumor protein p53, Antigen NY-CO-13
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC003596
Gene Symbol P53
Gene ID (NCBI) 7157
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP04637
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

P53 is a 53 kDa protein that is activated in response to alteration of normal cell homeostasis, including DNA damage, nutrient starvation, heat shock, virus infection, pH change, hypoxia, and oncogene activation. P53 maintains genetic stability by regulating different processes, such as cell-cycle arrest, DNA synthesis and repair, programmed cell death, and energy metabolism. In non-stressed conditions these proteins bind p53, ubiquitylate it and target it for degradation by the proteasome. In stressed conditions the function of the MdM2-MdM4 complex is blocked by phosphorylation, protein-binding events and/or enhanced degradation. (PMID: 19935675, PMID: 24379683)

实验方案

Product Specific Protocols
IF protocol for CL594 Phospho-P53 (Ser15) antibody CL594-80195Download protocol
Standard Protocols
Click here to view our Standard Protocols