• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of HEK-293T using 81491-1-RR (same clone as 81491-1-PBS)

    WB analysis of HEK-293T using 81491-1-RR (same clone as 81491-1-PBS)

    Non-treated HEK-293T cells, phosphatase inhibitor treated and λ phosphatase HEK-293T cells were subjected to SDS PAGE followed by western blot with 81491-1-RR (Phospho-PDH E1 Alpha (Ser232) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 81491-1-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293T using 81491-1-RR (same clone as 81491-1-PBS)

    FC experiment of HEK-293T using 81491-1-RR (same clone as 81491-1-PBS)

    1X10^6 HEK-293T cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.06 ug Phospho-PDH E1 Alpha (Ser232) Recombinant antibody (81491-1-RR, Clone:1L22) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 81491-1-PBS in a different storage buffer formulation.

Phospho-PDH E1 Alpha (Ser232) Recombinant antibody, PBS Only

Phospho-PDH E1 Alpha (Ser232) Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 81491-1-PBS

产品说明书

CloneNo. 1L22

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), Indirect ELISA

别名

p PDH E1 Alpha Ser232, p PDH E1 Alpha (Ser232), p PDH E1 Alpha, PDH E1 Alpha (Ser232), Phospho PDH E1 Alpha

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy PDHA1 Ready-To-Use IHC Kit. PDHA1 primary antibody included.

产品信息

81491-1-PBS targets Phospho-PDH E1 Alpha (Ser232) in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 pyruvate dehydrogenase (lipoamide) alpha 1
别名 p PDH E1 Alpha Ser232, p PDH E1 Alpha (Ser232), p PDH E1 Alpha, PDH E1 Alpha (Ser232), Phospho PDH E1 Alpha
计算分子量 43 kDa
观测分子量 40 kDa
GenBank蛋白编号BC002406
基因名称 PDHA1
Gene ID (NCBI) 5160
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP08559
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PDH E1 Alpha (PDHA1), as the major component of PDH, can be phosphorylated and inactivated by PDHK1. PDHA1 contains three serine residues (Ser232, Ser293, or Ser300) that can be reversibly phosphorylated by a dedicated family of four inhibitory pyruvate dehydrogenase kinases (PDHK1-4) and two reactivating phosphatases (PDP1, 2). Hypoxia induces the expression of PDHK1 and PDHK3 and hyperphosphorylates PDHA1. Phosphorylated PDHA1 inactivates the whole pyruvate dehydrogenase complex (PDC), reduces pyruvate entering into the tricarboxylic acid (TCA) cycle for oxidative phosphorylation, enhances the Warburg effect and promotes tumorigenesis. (PMID: 30993888, PMID: 34749809)