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验证数据展示

  • WB analysis of HEK-293T using 81491-1-RR

    WB analysis of HEK-293T using 81491-1-RR

    Non-treated HEK-293T cells, phosphatase inhibitor treated and λ phosphatase HEK-293T cells were subjected to SDS PAGE followed by western blot with 81491-1-RR (Phospho-PDH E1 Alpha (Ser232) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

  • FC experiment of HEK-293T using 81491-1-RR

    FC experiment of HEK-293T using 81491-1-RR

    1X10^6 HEK-293T cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.06 ug Phospho-PDH E1 Alpha (Ser232) Recombinant antibody (81491-1-RR, Clone:1L22) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Phospho-PDH E1 Alpha (Ser232) Recombinant antibody

Phospho-PDH E1 Alpha (Ser232) Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 81491-1-RR

产品说明书

CloneNo. 1L22

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

p PDH E1 Alpha Ser232, p PDH E1 Alpha (Ser232), p PDH E1 Alpha, PDH E1 Alpha (Ser232), Phospho PDH E1 Alpha

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, λ phosphatase HEK-293T cells
Positive FC (Intra) detected inλ phosphatase treated HEK-293T cells
Planning an IHC experiment? We recommend our IHCeasy PDHA1 Ready-To-Use IHC Kit. PDHA1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.06 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81491-1-RR targets Phospho-PDH E1 Alpha (Ser232) in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 pyruvate dehydrogenase (lipoamide) alpha 1
别名 p PDH E1 Alpha Ser232, p PDH E1 Alpha (Ser232), p PDH E1 Alpha, PDH E1 Alpha (Ser232), Phospho PDH E1 Alpha
计算分子量 43 kDa
观测分子量 40 kDa
GenBank蛋白编号BC002406
基因名称 PDHA1
Gene ID (NCBI) 5160
RRIDAB_2935428
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP08559
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PDH E1 Alpha (PDHA1), as the major component of PDH, can be phosphorylated and inactivated by PDHK1. PDHA1 contains three serine residues (Ser232, Ser293, or Ser300) that can be reversibly phosphorylated by a dedicated family of four inhibitory pyruvate dehydrogenase kinases (PDHK1-4) and two reactivating phosphatases (PDP1, 2). Hypoxia induces the expression of PDHK1 and PDHK3 and hyperphosphorylates PDHA1. Phosphorylated PDHA1 inactivates the whole pyruvate dehydrogenase complex (PDC), reduces pyruvate entering into the tricarboxylic acid (TCA) cycle for oxidative phosphorylation, enhances the Warburg effect and promotes tumorigenesis. (PMID: 30993888, PMID: 34749809)

实验方案

Product Specific Protocols
WB protocol for Phospho-PDH E1 Alpha (Ser232) antibody 81491-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols