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验证数据展示

  • WB analysis of HEK-293T using 81491-1-RR

    WB analysis of HEK-293T using 81491-1-RR

    Non-treated HEK-293T cells, phosphatase inhibitor treated and λ phosphatase HEK-293T cells were subjected to SDS PAGE followed by western blot with 81491-1-RR (Phospho-PDH E1 Alpha (Ser232) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

  • FC experiment of HEK-293T using 81491-1-RR

    FC experiment of HEK-293T using 81491-1-RR

    1X10^6 HEK-293T cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.06 ug Phospho-PDH E1 Alpha (Ser232) Recombinant antibody (81491-1-RR, Clone:1L22) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Phospho-PDH E1 Alpha (Ser232) Recombinant antibody

Phospho-PDH E1 Alpha (Ser232) Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 81491-1-RR

Print datasheet

CloneNo. 1L22

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

p PDH E1 Alpha Ser232, p PDH E1 Alpha (Ser232), p PDH E1 Alpha, PDH E1 Alpha (Ser232), Phospho PDH E1 Alpha

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, λ phosphatase HEK-293T cells
Positive FC (Intra) detected inλ phosphatase treated HEK-293T cells
Planning an IHC experiment? We recommend our IHCeasy PDHA1 Ready-To-Use IHC Kit. PDHA1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.06 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81491-1-RR targets Phospho-PDH E1 Alpha (Ser232) in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name pyruvate dehydrogenase (lipoamide) alpha 1
Synonyms p PDH E1 Alpha Ser232, p PDH E1 Alpha (Ser232), p PDH E1 Alpha, PDH E1 Alpha (Ser232), Phospho PDH E1 Alpha
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC002406
Gene Symbol PDHA1
Gene ID (NCBI) 5160
RRIDAB_2935428
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08559
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PDH E1 Alpha (PDHA1), as the major component of PDH, can be phosphorylated and inactivated by PDHK1. PDHA1 contains three serine residues (Ser232, Ser293, or Ser300) that can be reversibly phosphorylated by a dedicated family of four inhibitory pyruvate dehydrogenase kinases (PDHK1-4) and two reactivating phosphatases (PDP1, 2). Hypoxia induces the expression of PDHK1 and PDHK3 and hyperphosphorylates PDHA1. Phosphorylated PDHA1 inactivates the whole pyruvate dehydrogenase complex (PDC), reduces pyruvate entering into the tricarboxylic acid (TCA) cycle for oxidative phosphorylation, enhances the Warburg effect and promotes tumorigenesis. (PMID: 30993888, PMID: 34749809)

实验方案

Product Specific Protocols
WB protocol for Phospho-PDH E1 Alpha (Ser232) antibody 81491-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols