• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Phospho-PERK/EIF2AK3 (Thr982) Recombinant antibody, PBS Only

Phospho-PERK/EIF2AK3 (Thr982) Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4E16

Cat No : 82534-1-PBS

Print datasheet

Synonyms

EIF2AK3, HRI, HsPEK, PERK, Phospho-PERK/EIF2AK3 (Thr980) mouse

验证数据展示

  • WB analysis of HEK-293 using 82534-1-RR (same clone as 82534-1-PBS)

    WB analysis of HEK-293 using 82534-1-RR (same clone as 82534-1-PBS)

    Non-treated HEK-293 cells and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 82534-1-RR (Phospho-PERK/EIF2AK3 (Thr982) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with beta actin antibody (66009-1-Ig) as loading control. This data was developed using the same antibody clone with 82534-1-PBS in a different storage buffer formulation.

  • WB analysis using 82534-1-RR (same clone as 82534-1-PBS)

    WB analysis using 82534-1-RR (same clone as 82534-1-PBS)

    Non-treated NIH/3T3 cells and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 82534-1-RR (Phospho-PERK/EIF2AK3 (Thr982) antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82534-1-PBS in a different storage buffer formulation.

This antibody is equivalent to Thr980 in mice.

产品信息

82534-1-PBS targets Phospho-PERK/EIF2AK3 (Thr982) in WB, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name eukaryotic translation initiation factor 2-alpha kinase 3
Synonyms EIF2AK3, HRI, HsPEK, PERK, Phospho-PERK/EIF2AK3 (Thr980) mouse
Calculated Molecular Weight 1116 aa, 125 kDa
Observed Molecular Weight180 kDa
GenBank Accession NumberBC126354
Gene Symbol EIF2AK3
Gene ID (NCBI) 9451
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NZJ5
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

EIF2AK3 encodes the protein kinase RNA-like ER kinase (PERK), a key regulator of the unfolded protein response (UPR) in response to ER stress. Under ER stress conditions, activation of PERK is triggered by the dissociation of glucose-regulated protein (GRP) 78 (also known as BiP) from its luminal domain, followed by oligomerization and autophosphorylation. Phosphorylated PERK subsequently phosphorylates eukaryotic translation initiation factor 2 alpha (eif2α), to attenuate global protein translation and reduce incoming ER protein load via upregulated ER chaperone expression. (PMID: 35922637, PMID: 32029570)