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Phospho-PERK/EIF2AK3 (Thr982) Recombinant antibody, PBS Only

Phospho-PERK/EIF2AK3 (Thr982) Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4E16

Cat no : 82534-1-PBS

Print datasheet

Synonyms

EIF2AK3, HRI, HsPEK, PERK, Phospho-PERK/EIF2AK3 (Thr980) mouse

验证数据展示

  • WB analysis of HEK-293 using 82534-1-RR (same clone as 82534-1-PBS)

    WB analysis of HEK-293 using 82534-1-RR (same clone as 82534-1-PBS)

    Non-treated HEK-293 cells and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 82534-1-RR (Phospho-PERK/EIF2AK3 (Thr982) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with beta actin antibody (66009-1-Ig) as loading control. This data was developed using the same antibody clone with 82534-1-PBS in a different storage buffer formulation.

  • WB analysis using 82534-1-RR (same clone as 82534-1-PBS)

    WB analysis using 82534-1-RR (same clone as 82534-1-PBS)

    Non-treated NIH/3T3 cells and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 82534-1-RR (Phospho-PERK/EIF2AK3 (Thr982) antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82534-1-PBS in a different storage buffer formulation.

This antibody is equivalent to Thr980 in mice.

产品信息

82534-1-PBS targets Phospho-PERK/EIF2AK3 (Thr982) in WB, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name eukaryotic translation initiation factor 2-alpha kinase 3
Synonyms EIF2AK3, HRI, HsPEK, PERK, Phospho-PERK/EIF2AK3 (Thr980) mouse
Calculated Molecular Weight 1116 aa, 125 kDa
Observed Molecular Weight180 kDa
GenBank Accession NumberBC126354
Gene Symbol PERK
Gene ID (NCBI) 9451
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NZJ5
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EIF2AK3 encodes the protein kinase RNA-like ER kinase (PERK), a key regulator of the unfolded protein response (UPR) in response to ER stress. Under ER stress conditions, activation of PERK is triggered by the dissociation of glucose-regulated protein (GRP) 78 (also known as BiP) from its luminal domain, followed by oligomerization and autophosphorylation. Phosphorylated PERK subsequently phosphorylates eukaryotic translation initiation factor 2 alpha (eif2α), to attenuate global protein translation and reduce incoming ER protein load via upregulated ER chaperone expression. (PMID: 35922637, PMID: 32029570)