Phospho-PERK/EIF2AK3 (Thr982) Recombinant antibody
Phospho-PERK/EIF2AK3 (Thr982) Uni-rAbTM Recombinant Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse and More (1)
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
4E16
验证数据展示
经过测试的应用
| Positive WB detected in | Calyculin A treated HEK-293 cells, Calyculin A treated NIH/3T3 cells |
This antibody is equivalent to Thr980 in mice.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:11200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
82534-1-RR targets Phospho-PERK/EIF2AK3 (Thr982) in WB, ELISA applications and shows reactivity with Human, mouse samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | Human, mouse |
| Cited Reactivity | human |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | eukaryotic translation initiation factor 2-alpha kinase 3 |
| Synonyms | EIF2AK3, HRI, HsPEK, PERK, Phospho-PERK/EIF2AK3 (Thr980) mouse |
| Calculated Molecular Weight | 1116 aa, 125 kDa |
| Observed Molecular Weight | 180 kDa |
| GenBank Accession Number | BC126354 |
| Gene Symbol | PERK |
| Gene ID (NCBI) | 9451 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9NZJ5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
EIF2AK3 encodes the protein kinase RNA-like ER kinase (PERK), a key regulator of the unfolded protein response (UPR) in response to ER stress. Under ER stress conditions, activation of PERK is triggered by the dissociation of glucose-regulated protein (GRP) 78 (also known as BiP) from its luminal domain, followed by oligomerization and autophosphorylation. Phosphorylated PERK subsequently phosphorylates eukaryotic translation initiation factor 2 alpha (eif2α), to attenuate global protein translation and reduce incoming ER protein load via upregulated ER chaperone expression. (PMID: 35922637, PMID: 32029570)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-PERK/EIF2AK3 (Thr982) antibody 82534-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Vet Microbiol Bovine parainfluenza virus type 3 infections induce ER stress-mediated autophagy to facilitate virus replication |