CoraLite® Plus 488-conjugated Phospho-PERK/EIF2AK3 (Thr982) Recombinant antibody

Phospho-PERK/EIF2AK3 (Thr982) Uni-rAbTM Recombinant Antibody for FC (Intra)
Cat No. CL488-82534

产品说明书

CloneNo. 4E16

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

FC (Intra)

PERK/EIF2AK3 (Thr982), Phospho PERK/EIF2AK3, Phospho PERK/EIF2AK3 (Thr982), Phospho-PERK/EIF2AK3, Phospho-PERK/EIF2AK3 Thr982

缓冲液配方:  PBS and Azide
PBS and Azide
规格: 

-/ -


经过测试的应用

Positive FC (Intra) detected inCalyculin A treated HEK-293 cells

This antibody is equivalent to Thr980 in mice.

For other applications, we recommend the unconjugated version of this antibody, 82534-1-PBS

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-82534 targets Phospho-PERK/EIF2AK3 (Thr982) in FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 eukaryotic translation initiation factor 2-alpha kinase 3
别名 PERK/EIF2AK3 (Thr982), Phospho PERK/EIF2AK3, Phospho PERK/EIF2AK3 (Thr982), Phospho-PERK/EIF2AK3, Phospho-PERK/EIF2AK3 Thr982
计算分子量 1116 aa, 125 kDa
观测分子量180 kDa
GenBank蛋白编号BC126354
基因名称 PERK
Gene ID (NCBI) 9451
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NZJ5
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EIF2AK3 encodes the protein kinase RNA-like ER kinase (PERK), a key regulator of the unfolded protein response (UPR) in response to ER stress. Under ER stress conditions, activation of PERK is triggered by the dissociation of glucose-regulated protein (GRP) 78 (also known as BiP) from its luminal domain, followed by oligomerization and autophosphorylation. Phosphorylated PERK subsequently phosphorylates eukaryotic translation initiation factor 2 alpha (eif2α), to attenuate global protein translation and reduce incoming ER protein load via upregulated ER chaperone expression. (PMID: 35922637, PMID: 32029570)

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