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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 81575-2-RR (same clone as 81575-2-PBS)

    WB analysis using 81575-2-RR (same clone as 81575-2-PBS)

    Non-treated THP-1 cells, phosphatase inhibitor treated THP-1 cells and λ phosphatase treated THP-1 cells were subjected to SDS PAGE followed by western blot with 81575-2-RR (Phospho-PKC Alpha (Thr638) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 81575-2-PBS in a different storage buffer formulation.

Phospho-PKC Alpha (Thr638) Recombinant antibody, PBS Only

Phospho-PKC Alpha (Thr638) Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 81575-2-PBS

Print datasheet

CloneNo. 241844A10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

PKC alpha, PRKCA, PKCA, PKC A, EC:2.7.11.13

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PRKCA Ready-To-Use IHC Kit. PRKCA primary antibody included.

产品信息

81575-2-PBS targets Phospho-PKC Alpha (Thr638) in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name protein kinase C, alpha
Synonyms PKC alpha, PRKCA, PKCA, PKC A, EC:2.7.11.13
Calculated Molecular Weight 77 kDa
Observed Molecular Weight77 kDa
GenBank Accession NumberAK055431
Gene Symbol PKC Alpha
Gene ID (NCBI) 5578
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP17252
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PKCs are a family of serine/threonine kinases involved in various processes in cells including proliferation, differentiation, cell survival, and apoptosis. PKC family is composed of three different subgroups: conventional (cPKC), the novel (nPKC) and atypical (aPKC). PKCα, β1, β2, and γ belong to cPKC, PKCδ, ε, η and θ are nPKC, whereas aPKC comprises of PKCζ and λ/ι. All PKCs consist of the N-terminal regulatory region and C-terminal catalytic region (kinase domain). PKCs are physiologically activated by various extracellular signals transduced by hormones, growth factors, cytokines or antigens. The presence of activated PKCs on internal membranes leads to the phosphorylation of various interacting proteins. (PMID: 32466765, PMID: 12417016)