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Conjugate
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验证数据展示

  • WB analysis using 28925-1-AP (same clone as 28925-1-PBS)

    WB analysis using 28925-1-AP (same clone as 28925-1-PBS)

    Non-treated HeLa and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 28925-1-AP (Phospo-PLA2G4A (Ser505) antibody) at dilution of 1:3000 incubated at 4℃ overnight. This data was developed using the same antibody clone with 28925-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 28925-1-AP (same clone as 28925-1-PBS)

    IF Staining of HeLa using 28925-1-AP (same clone as 28925-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed λ phosphatase treated HeLa cells using Phospho-PLA2G4A (Ser505) antibody (28925-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 28925-1-PBS in a different storage buffer formulation.

Phospho-PLA2G4A (Ser505) Polyclonal antibody, PBS Only

Phospho-PLA2G4A (Ser505) Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 28925-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

28925-1-PBS targets Phospho-PLA2G4A (Ser505) in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phospholipase A2, group IVA (cytosolic, calcium-dependent)
别名
计算分子量 749 aa, 85 kDa
观测分子量100-110 kDa
GenBank蛋白编号BC114340
基因名称 PLA2G4A
Gene ID (NCBI) 5321
RRIDAB_2918213
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP47712
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PLA2G4A, also named as cPLA2 (cytosolic phospholipase A2), is a highly conserved and widely expressed enzyme which can promote lipid mediator production. PLA2G4A plays a major role in membrane lipid remodeling and biosynthesis of lipid mediators of the inflammatory response. It has been reported that the activity and expression levels of PLA2G4A are upregulated in several neurodegenerative diseases. PLA2G4A can be activated at the phosphorylation site of ser505 by MAP kinase. (PMID: 25838312, 8381049, 31238788)