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CoraLite® Plus 647-conjugated Phospho-RPS6KA1 (Ser380) Recombinant antibody
Phospho-RPS6KA1 (Ser380) Uni-rAbTM Recombinant Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
FC (Intra)
Conjugate
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
7F23
验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | TPA treated Jurkat cells |
Planning an IHC experiment? We recommend our IHCeasy Phospho-RPS6KA1 (Ser380) Ready-To-Use IHC Kit. Phospho-RPS6KA1 (Ser380) primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 80108-1-PBS
推荐稀释比
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL647-80108 targets Phospho-RPS6KA1 (Ser380) in FC (Intra) applications and shows reactivity with human, mouse samples.
| Tested Applications | FC (Intra) Application Description |
| Tested Reactivity | human, mouse |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | ribosomal protein S6 kinase, 90kDa, polypeptide 1 |
| Synonyms | RPS6KA1, MAPKAP kinase 1a, MAPK-activated protein kinase 1a, MAP kinase-activated protein kinase 1a, HU 1 |
| Calculated Molecular Weight | 735 aa, 83 kDa |
| Observed Molecular Weight | 83-90 kDa |
| GenBank Accession Number | BC014966 |
| Gene Symbol | RPS6KA1 |
| Gene ID (NCBI) | 6195 |
| RRID | AB_2920329 |
| Conjugate | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q15418 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed serine/threonine kinases and play an important role in the MAPK signaling cascade and act downstream of ERK1/2 signaling. It can mediate mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77. RSK1 (RPS6KA1) is also designated as p90RSK associated with cellular proliferation, survival and differentiation. It has been reported that the phosphorylation of RPS6KA1 at Ser380 is involved in autophosphorylation by activation of the C-terminal kinase domain. (PMID: 16458888, 30813401, 17259979, 10679322)