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验证数据展示

  • WB analysis of THP-1 using 82674-10-RR

    WB analysis of THP-1 using 82674-10-RR

    Non-treated THP-1 cells and IFN gamma treated THP-1 cells were subjected to SDS PAGE followed by western blot with 82674-10-RR (Phospho-STAT1 (Tyr701) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as loading control.

  • FC experiment of THP-1 using 82674-10-RR

    FC experiment of THP-1 using 82674-10-RR

    1x10^6 IFN gamma treated THP-1 cells were intracellularly stained with 0.13 ug Phospho-STAT1 (Tyr701) Recombinant antibody (82674-10-RR, Clone:1C5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Phospho-STAT1 (Tyr701) Recombinant antibody

Phospho-STAT1 (Tyr701) Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 82674-10-RR

产品说明书

CloneNo. 1C5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

STAT1, Transcription factor ISGF-3 components p91/p84, STAT91, Signal transducer and activator of transcription 1-alpha/beta, ISGF 3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inIFN gamma treated THP-1 cells
Positive FC (Intra) detected inIFN gamma treated THP-1 cells
Planning an IHC experiment? We recommend our IHCeasy STAT1 Ready-To-Use IHC Kit. STAT1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82674-10-RR targets Phospho-STAT1 (Tyr701) in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 signal transducer and activator of transcription 1, 91kDa
别名 STAT1, Transcription factor ISGF-3 components p91/p84, STAT91, Signal transducer and activator of transcription 1-alpha/beta, ISGF 3
计算分子量 83 kDa
观测分子量84-91 kDa
GenBank蛋白编号BC002704
基因名称 STAT1
Gene ID (NCBI) 6772
RRIDAB_3085362
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP42224
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STAT1 (signal transducers and activators of transcription 1) is a member of the STAT protein family. STAT family members are phosphorylated by the receptor associated kinases, and then form homo-or heterodimers that translocate to the cell nucleus where they act as transcription activators. The phosphorylation of STAT1 at Tyr701 promotes the dimerization of STAT1 and its translocation into the nucleus where it binds the GAS promoter to allow the transcription of inflammatory response genes(PMID: 31408005). The transcript of STAT1 undergoes alternative splicing, resulting in two isoforms: STAT1α (91 kDa) and STAT1β (84 kDa)(PMID: 20159032).

实验方案

Product Specific Protocols
WB protocol for Phospho-STAT1 (Tyr701) antibody 82674-10-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols