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Phospho-STAT1 (Tyr701) Recombinant antibody

Phospho-STAT1 (Tyr701) Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1C5

Cat no : 82674-10-RR

Print datasheet

Synonyms

STAT1, Transcription factor ISGF-3 components p91/p84, STAT91, Signal transducer and activator of transcription 1-alpha/beta, ISGF 3

验证数据展示

  • WB analysis of THP-1 using 82674-10-RR

    WB analysis of THP-1 using 82674-10-RR

    Non-treated THP-1 cells and IFN gamma treated THP-1 cells were subjected to SDS PAGE followed by western blot with 82674-10-RR (Phospho-STAT1 (Tyr701) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as loading control.

  • FC experiment of THP-1 using 82674-10-RR

    FC experiment of THP-1 using 82674-10-RR

    1x10^6 IFN gamma treated THP-1 cells were intracellularly stained with 0.13 ug Phospho-STAT1 (Tyr701) Recombinant antibody (82674-10-RR, Clone:1C5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

经过测试的应用

Positive WB detected inIFN gamma treated THP-1 cells
Positive FC (Intra) detected inIFN gamma treated THP-1 cells
Planning an IHC experiment? We recommend our IHCeasy STAT1 Ready-To-Use IHC Kit. STAT1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82674-10-RR targets Phospho-STAT1 (Tyr701) in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name signal transducer and activator of transcription 1, 91kDa
Synonyms STAT1, Transcription factor ISGF-3 components p91/p84, STAT91, Signal transducer and activator of transcription 1-alpha/beta, ISGF 3
Calculated Molecular Weight 83 kDa
Observed Molecular Weight84-91 kDa
GenBank Accession NumberBC002704
Gene Symbol STAT1
Gene ID (NCBI) 6772
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP42224
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STAT1 (signal transducers and activators of transcription 1) is a member of the STAT protein family. STAT family members are phosphorylated by the receptor associated kinases, and then form homo-or heterodimers that translocate to the cell nucleus where they act as transcription activators. The phosphorylation of STAT1 at Tyr701 promotes the dimerization of STAT1 and its translocation into the nucleus where it binds the GAS promoter to allow the transcription of inflammatory response genes(PMID: 31408005). The transcript of STAT1 undergoes alternative splicing, resulting in two isoforms: STAT1α (91 kDa) and STAT1β (84 kDa)(PMID: 20159032).

实验方案

Product Specific Protocols
WB protocol for Phospho-STAT1 (Tyr701) antibody 82674-10-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols