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验证数据展示

  • WB analysis using 80127-1-RR (same clone as 80127-1-PBS)

    WB analysis using 80127-1-RR (same clone as 80127-1-PBS)

    Non-treated PC-3 and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 80127-1-RR (Phospho-STK11/LKB1 (Thr189) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 80127-1-PBS in a different storage buffer formulation.

  • IF Staining of PC-3 using 80127-1-RR (same clone as 80127-1-PBS)

    IF Staining of PC-3 using 80127-1-RR (same clone as 80127-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed non-treated PC-3 cells and Calyculin A treated PC-3 cells using Phospho-STK11/LKB1 (Thr189) antibody (80127-1-RR, Clone: 5J9 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80127-1-PBS in a different storage buffer formulation.

  • FC experiment of PC-3 using 80127-1-RR (same clone as 80127-1-PBS)

    FC experiment of PC-3 using 80127-1-RR (same clone as 80127-1-PBS)

    1X10^6 PC-3 cells untreated (dashed lines) or treated with Calyculin A which intracellularly stained with 0.13 ug Phospho-STK11/LKB1 (Thr189) Recombinant monoclonal antibody (80127-1-RR, Clone:5J9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 80127-1-PBS in a different storage buffer formulation.

Phospho-STK11/LKB1 (Thr189) Recombinant monoclonal antibody, PBS Only

Phospho-STK11/LKB1 (Thr189) Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 80127-1-PBS

产品说明书

CloneNo. 5J9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, FC (Intra), Indirect ELISA

别名

p STK11, p STK11 (Thr189), p STK11 Thr189, p STK11/LKB1, p STK11/LKB1 (Thr189)

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy STK11 Ready-To-Use IHC Kit. STK11 primary antibody included.

产品信息

80127-1-PBS targets Phospho-STK11/LKB1 (Thr189) in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 serine/threonine kinase 11
别名 p STK11, p STK11 (Thr189), p STK11 Thr189, p STK11/LKB1, p STK11/LKB1 (Thr189)
计算分子量 49 kDa
观测分子量50-55 kDa
GenBank蛋白编号BC007981
基因名称 LKB1
Gene ID (NCBI) 6794
RRIDAB_2918867
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ15831
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

STK11(serine/threonine-protein kinase 11) is also named as LKB1, PJS, and belongs to the protein kinase superfamily. It controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. The tumour suppressor protein LKB1 is a serine/threonine kinase that has been causally linked to Peutz-Jeghers syndrome (PJS). Defects in STK11 are a cause of Peutz-Jeghers syndrome (PJS) and defects in STK11 have been associated with testicular germ cell tumor (TGCT) and some sporadic cancers, especially lung cancers.

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