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验证数据展示

  • WB analysis using 80127-1-RR

    WB analysis using 80127-1-RR

    Non-treated PC-3 and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 80127-1-RR (Phospho-STK11/LKB1 (Thr189) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

  • IF Staining of PC-3 using 80127-1-RR

    IF Staining of PC-3 using 80127-1-RR

    Immunofluorescent analysis of (4% PFA) fixed non-treated PC-3 cells and Calyculin A treated PC-3 cells using Phospho-STK11/LKB1 (Thr189) antibody (80127-1-RR, Clone: 5J9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • FC experiment of PC-3 using 80127-1-RR

    FC experiment of PC-3 using 80127-1-RR

    1X10^6 PC-3 cells untreated (dashed lines) or Calyculin A treated (red) were intracellularly stained with 0.25 ug Anti-Human Phospho-STK11 (Thr189) (80127-1-RR, Clone:5J9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.25 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Phospho-STK11/LKB1 (Thr189) Recombinant antibody

Phospho-STK11/LKB1 (Thr189) Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 80127-1-RR

产品说明书

CloneNo. 5J9
引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

Human, Mouse and More (1)

应用

WB, IF/ICC, FC (Intra), ELISA

别名

LKB1, STK11, STK11/ LKB1, PJS, Liver kinase B1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inPC-3 cells, HEK-293 cells, NIH/3T3 cells, Calyculin A treated HEK-293 cells, Calyculin A treated PC-3 cells, Calyculin A treated NIH/3T3 cells
Positive IF/ICC detected inCalyculin A treated PC-3 cells
Positive FC (Intra) detected inCalyculin A treated PC-3 cells
Planning an IHC experiment? We recommend our IHCeasy STK11 Ready-To-Use IHC Kit. STK11 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

80127-1-RR targets Phospho-STK11/LKB1 (Thr189) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with Human, Mouse samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
文献引用应用WB
经测试反应性 Human, Mouse
文献引用反应性mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 serine/threonine kinase 11
别名 LKB1, STK11, STK11/ LKB1, PJS, Liver kinase B1
计算分子量 49 kDa
观测分子量50-55 kDa
GenBank蛋白编号BC007981
基因名称 LKB1
Gene ID (NCBI) 6794
RRIDAB_2918867
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ15831
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STK11(serine/threonine-protein kinase 11) is also named as LKB1, PJS, and belongs to the protein kinase superfamily. It controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. The tumour suppressor protein LKB1 is a serine/threonine kinase that has been causally linked to Peutz-Jeghers syndrome (PJS). Defects in STK11 are a cause of Peutz-Jeghers syndrome (PJS) and defects in STK11 have been associated with testicular germ cell tumor (TGCT) and some sporadic cancers, especially lung cancers.

实验方案

Product Specific Protocols
WB protocol for Phospho-STK11/LKB1 (Thr189) antibody 80127-1-RRDownload protocol
IF protocol for Phospho-STK11/LKB1 (Thr189) antibody 80127-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

PLoS One

Chronic intermittent hypoxia aggravated diabetic cardiomyopathy through LKB1/AMPK/Nrf2 signaling pathway

Authors - Bingbing Liu
View Article