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验证数据展示

  • WB analysis using 80101-7-RR

    WB analysis using 80101-7-RR

    Non-treated SH-SY5Y cells, phosphatase inhibitor treated SH-SY5Y cells and λ phosphatase treated SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 80101-7-RR (Phospho-TAU (Thr231) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

  • FC experiment of SH-SY5Y using 80101-7-RR

    FC experiment of SH-SY5Y using 80101-7-RR

    1X10^6 SH-SY5Y cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.06 ug Phospho-TAU (Thr231) Recombinant antibody (80101-7-RR, Clone:240632B1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Phospho-TAU (Thr231) Recombinant antibody

Phospho-TAU (Thr231) Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 80101-7-RR

产品说明书

CloneNo. 240632B1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

p TAU, p TAU (Thr231), Phospho TAU, Phospho TAU (Thr231), Phospho-TAU

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inSH-SY5Y cells, λ phosphatase treated SH-SY5Y cells
Positive FC (Intra) detected inλ phosphatase treated SH-SY5Y cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.06 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80101-7-RR targets Phospho-TAU (Thr231) in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 microtubule-associated protein tau
别名 p TAU, p TAU (Thr231), Phospho TAU, Phospho TAU (Thr231), Phospho-TAU
计算分子量 37-46, 79-81 kDa
观测分子量50-80 kDa
GenBank蛋白编号BC000558
基因名称 TAU
Gene ID (NCBI) 4137
RRIDAB_3670466
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP10636
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tau (tubulin-associated unit) is a microtubule-associated protein (also known as MAPT), expressed mainly in neurons of the central nervous system. Its primary function is to modulate microtubule dynamics for maintaining axonal cytoskeleton. The Tau protein has six isoforms produced from a single gene through alternative RNA splicing. Isoforms differ in number of inserts at the N-terminal half and the number of repeats at the C-terminal half (3 repeat-3R; 4 repeat-4R). Tau is hyperphosphorylated during aging and in age-related neurodegenerative diseases such as Alzheimer's disease (AD) and fronto-temporal dementia. Hyperphosphorylation of Tau leads to the formation of neurofibrillary tangles (NFT) in the neurons and glia cells, which is one of the hallmarks of AD.

实验方案

Product Specific Protocols
WB protocol for Phospho-TAU (Thr231) antibody 80101-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols