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验证数据展示

  • WB analysis using 80101-7-RR

    WB analysis using 80101-7-RR

    Non-treated SH-SY5Y cells, phosphatase inhibitor treated SH-SY5Y cells and λ phosphatase treated SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 80101-7-RR (Phospho-TAU (Thr231) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

  • FC experiment of SH-SY5Y using 80101-7-RR

    FC experiment of SH-SY5Y using 80101-7-RR

    1X10^6 SH-SY5Y cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.06 ug Phospho-TAU (Thr231) Recombinant antibody (80101-7-RR, Clone:240632B1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Phospho-TAU (Thr231) Recombinant antibody

Phospho-TAU (Thr231) Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 80101-7-RR

Print datasheet

CloneNo. 240632B1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

p TAU, p TAU (Thr231), Phospho TAU, Phospho TAU (Thr231), Phospho-TAU

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inSH-SY5Y cells, λ phosphatase treated SH-SY5Y cells
Positive FC (Intra) detected inλ phosphatase treated SH-SY5Y cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.06 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80101-7-RR targets Phospho-TAU (Thr231) in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name microtubule-associated protein tau
Synonyms p TAU, p TAU (Thr231), Phospho TAU, Phospho TAU (Thr231), Phospho-TAU
Calculated Molecular Weight 37-46, 79-81 kDa
Observed Molecular Weight50-80 kDa
GenBank Accession NumberBC000558
Gene Symbol TAU
Gene ID (NCBI) 4137
RRIDAB_3670466
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP10636
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tau (tubulin-associated unit) is a microtubule-associated protein (also known as MAPT), expressed mainly in neurons of the central nervous system. Its primary function is to modulate microtubule dynamics for maintaining axonal cytoskeleton. The Tau protein has six isoforms produced from a single gene through alternative RNA splicing. Isoforms differ in number of inserts at the N-terminal half and the number of repeats at the C-terminal half (3 repeat-3R; 4 repeat-4R). Tau is hyperphosphorylated during aging and in age-related neurodegenerative diseases such as Alzheimer's disease (AD) and fronto-temporal dementia. Hyperphosphorylation of Tau leads to the formation of neurofibrillary tangles (NFT) in the neurons and glia cells, which is one of the hallmarks of AD.

实验方案

Product Specific Protocols
WB protocol for Phospho-TAU (Thr231) antibody 80101-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols