Phospho-mTOR (Ser2448) Monoclonal antibody

Phospho-mTOR (Ser2448) Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

2A12G3

Cat no : 67778-1-Ig

Print datasheet

Synonyms

p mTOR, p mTOR (Ser2448), Phospho mTOR, Phospho mTOR (Ser2448), Phospho-mTOR



经过测试的应用

Positive WB detected inHeLa cells, NIH/3T3 cells, HEK-293T cells, HEK-293 cells, HSC-T6 cells, Calyculin A treated HeLa cells, EGF treated NIH/3T3 cells, Rapamycin treated HEK-293 cells, Calyculin A treated HEK-293 cells
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inCalyculin A treated HeLa cells, 100 nM Calyculin A (30 minutes) treated HeLa cells
Planning an IHC experiment? We recommend our IHCeasy MTOR Ready-To-Use IHC Kit. MTOR primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67778-1-Ig targets Phospho-mTOR (Ser2448) in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, chicken, bovine
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name FK506 binding protein 12-rapamycin associated protein 1
Synonyms p mTOR, p mTOR (Ser2448), Phospho mTOR, Phospho mTOR (Ser2448), Phospho-mTOR
Calculated Molecular Weight 289 kDa
Observed Molecular Weight 289 kDa
GenBank Accession NumberBC117166
Gene Symbol mTOR
Gene ID (NCBI) 2475
RRIDAB_2889842
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481. mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors.

实验方案

Product Specific Protocols
WB protocol for Phospho-mTOR (Ser2448) antibody 67778-1-IgDownload protocol
IHC protocol for Phospho-mTOR (Ser2448) antibody 67778-1-IgDownload protocol
IF protocol for Phospho-mTOR (Ser2448) antibody 67778-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Rep Med

Reprograming immunosuppressive microenvironment by eIF4G1 targeting to eradicate pancreatic ductal adenocarcinoma

Authors - Lihong He
humanWB

Autophagy

Periplocin suppresses the growth of colorectal cancer cells by triggering LGALS3 (galectin 3)-mediated lysophagy

Authors - Kui Wang
mouseWB

Redox Biol

GSTP1-mediated S-glutathionylation of Pik3r1 is a redox hub that inhibits osteoclastogenesis through regulating autophagic flux

Authors - Xiaoxiao Ji
humanWB

J Exp Clin Cancer Res

Circular RNA circATP9A promotes non-small cell lung cancer progression by interacting with HuR and by promoting extracellular vesicles-mediated macrophage M2 polarization

Authors - Yuanshan Yao
humanWB

J Control Release

cRGD-targeted heparin nanoparticles for effective dual drug treatment of cisplatin-resistant ovarian cancer

Authors - Xiaomei Liang
humanWB

Mater Today Bio

Carbon dots enhance extracellular matrix secretion for dentin-pulp complex regeneration through PI3K/Akt/mTOR pathway-mediated activation of autophagy.

Authors - Lili Liu