验证数据展示

WB analysis using 67778-1-Ig
Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 67778-1-Ig (Phospho-mTOR (Ser2448) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 67778-1-Ig
Non-treated and EGF treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67778-1-Ig (Phospho-mTOR (Ser2448) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 67778-1-Ig
Non-treated and Rapamycin treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 67778-1-Ig (Phospho-mTOR (Ser2448) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with RICTOR antibody (66867-2-Ig) and mTOR antibody (66888-1-Ig) subsequently.
WB analysis using 67778-1-Ig
Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 67778-1-Ig (Phospho-mTOR (Ser2448) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with RICTOR antibody (66867-2-Ig) and mTOR antibody (66888-1-Ig) subsequently.
IHC staining of human colon cancer using 67778-1-Ig
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67778-1-Ig (Phospho-mTOR (Ser2448) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with MTOR antibody included
IF Staining of HepG2 using 67778-1-Ig
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Phospho-mTOR (Ser2448) antibody (67778-1-Ig, Clone: 2A12G3 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
FC experiment of HeLa using 67778-1-Ig
1X10^6 Calyculin A treated HeLa cells were intracellularly stained with 0.5 ug Anti-Human Phospho-mTOR (Ser2448) (67778-1-Ig, Clone:2A12G3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with 90% MeOH.
FC experiment of HeLa using 67778-1-Ig
1x10^6 100 nM Calyculin A (30 minutes) treated HeLa cells were intracellularly stained with 0.2 μg Phospho-mTOR (Ser2448) Monoclonal antibody (67778-1-Ig, Clone:2A12G3) and CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(Cat.NO.RGAM005), and 0.2 μg KLH (66360-3-Ig, Clone: K11B8C4B5). Cells were fixed with 4% PFA .

Phospho-mTOR (Ser2448) Monoclonal antibody

Phospho-mTOR (Ser2448) Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 67778-1-Ig

产品说明书

CloneNo. 2A12G3

引用文献(315)

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat and More (3)

应用

WB, IHC, IF/ICC, FC (Intra), ELISA and More (1)

别名

p mTOR, p mTOR (Ser2448), Phospho mTOR, Phospho mTOR (Ser2448), Phospho-mTOR

缓冲液配方: PBS and Azide

PBS and Azide

PBS Only

偶联物: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 647 CoraLite®594

规格:

-/ -

点击咨询售前客服

点击咨询售后客服

概述

经过测试的应用
产品信息
发表文章 (315)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	HeLa cells, NIH/3T3 cells, HEK-293T cells, HEK-293 cells, HSC-T6 cells, Calyculin A treated HeLa cells, EGF treated NIH/3T3 cells, Rapamycin treated HEK-293 cells, Calyculin A treated HEK-293 cells
Positive IHC detected in	human colon cancer tissue, human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	Calyculin A treated HeLa cells, 100 nM Calyculin A (30 minutes) treated HeLa cells

Planning an IHC experiment? We recommend our IHCeasy MTOR Ready-To-Use IHC Kit. MTOR primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.

应用	推荐稀释比
Western Blot (WB)	WB : 1:2000-1:10000
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WB	See 305 publications below
IHC	See 21 publications below
IF	See 16 publications below

产品信息

67778-1-Ig targets Phospho-mTOR (Ser2448) in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
文献引用应用	WB, IHC, IF
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse, rat, pig, chicken, bovine
免疫原	Peptide 种属同源性预测
宿主/亚型	Mouse / IgG2b
抗体类别	Monoclonal
产品类型	Antibody
全称	FK506 binding protein 12-rapamycin associated protein 1
别名	p mTOR, p mTOR (Ser2448), Phospho mTOR, Phospho mTOR (Ser2448), Phospho-mTOR
计算分子量	289 kDa
观测分子量	289 kDa
GenBank蛋白编号	BC117166
基因名称	mTOR
Gene ID (NCBI)	2475
RRID	AB_2889842
偶联类型	Unconjugated
形式	Liquid
纯化方式	Protein A purification
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

MTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481. mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors.

实验方案

Product Specific Protocols
WB protocol for Phospho-mTOR (Ser2448) antibody 67778-1-Ig	Download protocol
IHC protocol for Phospho-mTOR (Ser2448) antibody 67778-1-Ig	Download protocol
IF protocol for Phospho-mTOR (Ser2448) antibody 67778-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB	Cell Rep Med Reprograming immunosuppressive microenvironment by eIF4G1 targeting to eradicate pancreatic ductal adenocarcinoma Authors - Lihong He View Article
human	WB	Autophagy Periplocin suppresses the growth of colorectal cancer cells by triggering LGALS3 (galectin 3)-mediated lysophagy Authors - Kui Wang View Article
mouse	WB	Redox Biol GSTP1-mediated S-glutathionylation of Pik3r1 is a redox hub that inhibits osteoclastogenesis through regulating autophagic flux Authors - Xiaoxiao Ji View Article
human	WB	J Exp Clin Cancer Res Circular RNA circATP9A promotes non-small cell lung cancer progression by interacting with HuR and by promoting extracellular vesicles-mediated macrophage M2 polarization Authors - Yuanshan Yao View Article
human	WB	J Control Release cRGD-targeted heparin nanoparticles for effective dual drug treatment of cisplatin-resistant ovarian cancer Authors - Xiaomei Liang View Article
human	WB	Mater Today Bio Carbon dots enhance extracellular matrix secretion for dentin-pulp complex regeneration through PI3K/Akt/mTOR pathway-mediated activation of autophagy. Authors - Lili Liu View Article

验证数据展示

WB analysis using 67778-1-Ig

WB analysis using 67778-1-Ig

WB analysis using 67778-1-Ig

WB analysis using 67778-1-Ig

IHC staining of human colon cancer using 67778-1-Ig

IF Staining of HepG2 using 67778-1-Ig

FC experiment of HeLa using 67778-1-Ig

FC experiment of HeLa using 67778-1-Ig

Phospho-mTOR (Ser2448) Monoclonal antibody

Phospho-mTOR (Ser2448) Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

-/ -

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Reprograming immunosuppressive microenvironment by eIF4G1 targeting to eradicate pancreatic ductal adenocarcinoma

Periplocin suppresses the growth of colorectal cancer cells by triggering LGALS3 (galectin 3)-mediated lysophagy

GSTP1-mediated S-glutathionylation of Pik3r1 is a redox hub that inhibits osteoclastogenesis through regulating autophagic flux

Circular RNA circATP9A promotes non-small cell lung cancer progression by interacting with HuR and by promoting extracellular vesicles-mediated macrophage M2 polarization

cRGD-targeted heparin nanoparticles for effective dual drug treatment of cisplatin-resistant ovarian cancer

Carbon dots enhance extracellular matrix secretion for dentin-pulp complex regeneration through PI3K/Akt/mTOR pathway-mediated activation of autophagy.