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验证数据展示

  • IF Staining of HepG2 using CL488-67778

    IF Staining of HepG2 using CL488-67778

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 Phospho-mTOR (Ser2448) antibody (CL488-67778, Clone: 2A12G3 ) at dilution of 1:100. DAPI (blue)

  • FC experiment of HeLa using CL488-67778

    FC experiment of HeLa using CL488-67778

    1X10^6 Calyculin A treated HeLa cells were intracellularly stained with 0.25 ug CoraLite® Plus 488 Anti-Human Phospho-mTOR (Ser2448) (CL488-67778, Clone:2A12G3) (red), or 0.25 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

CoraLite® Plus 488-conjugated Phospho-mTOR (Ser2448) Monoclonal antibody

Phospho-mTOR (Ser2448) Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67778

产品说明书

CloneNo. 2A12G3

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse

应用

IF/ICC, FC (Intra)

别名

FRAP1, MTOR, FRAP2, FRAP, FKBP12-rapamycin complex-associated protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inCalyculin A treated HeLa cells
Planning an IHC experiment? We recommend our IHCeasy MTOR Ready-To-Use IHC Kit. MTOR primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67778-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67778 targets Phospho-mTOR (Ser2448) in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 FK506 binding protein 12-rapamycin associated protein 1
别名 FRAP1, MTOR, FRAP2, FRAP, FKBP12-rapamycin complex-associated protein
计算分子量 289 kDa
GenBank蛋白编号BC117166
基因名称 mTOR
Gene ID (NCBI) 2475
RRIDAB_2919545
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481. mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Phospho-mTOR (Ser2448) antibody CL488-67778Download protocol
Standard Protocols
Click here to view our Standard Protocols