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验证数据展示

  • WB analysis using 80596-1-RR (same clone as 80596-1-PBS)

    WB analysis using 80596-1-RR (same clone as 80596-1-PBS)

    Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80596-1-RR (Phospho-mTOR (Ser2448) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with RICTOR antibody (66867-2-Ig) subsequently. This data was developed using the same antibody clone with 80596-1-PBS in a different storage buffer formulation.

  • WB analysis using 80596-1-RR (same clone as 80596-1-PBS)

    WB analysis using 80596-1-RR (same clone as 80596-1-PBS)

    Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 80596-1-RR (Phospho-mTOR (Ser2448) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with RICTOR antibody (66867-2-Ig) subsequently. This data was developed using the same antibody clone with 80596-1-PBS in a different storage buffer formulation.

  • WB analysis of HSC-T6 using 80596-1-RR (same clone as 80596-1-PBS)

    WB analysis of HSC-T6 using 80596-1-RR (same clone as 80596-1-PBS)

    Calyculin A treated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 80596-1-RR (Phospho-mTOR (Ser2448) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80596-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 80596-1-RR (same clone as 80596-1-PBS)

    IF Staining of HEK-293 using 80596-1-RR (same clone as 80596-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed PMA treated HEK-293 cells using Phospho-mTOR (Ser2448) antibody (80596-1-RR, Clone: 3L18 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 80596-1-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 using 80596-1-RR (same clone as 80596-1-PBS)

    FC experiment of HEK-293 using 80596-1-RR (same clone as 80596-1-PBS)

    1X10^6 HEK-293 cells untreated (dashed lines) or treated with Calyculin A which intracellularly stained with 0.13 ug Phospho-mTOR (Ser2448) Recombinant antibody (80596-1-RR, Clone:3L18) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 80596-1-PBS in a different storage buffer formulation.

Phospho-mTOR (Ser2448) Recombinant antibody, PBS Only

Phospho-mTOR (Ser2448) Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 80596-1-PBS

产品说明书

CloneNo. 3L18

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, FC (Intra), Indirect ELISA

别名

FRAP1, MTOR, FRAP2, FRAP, FKBP12-rapamycin complex-associated protein

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy MTOR Ready-To-Use IHC Kit. MTOR primary antibody included.

产品信息

80596-1-PBS targets Phospho-mTOR (Ser2448) in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 FK506 binding protein 12-rapamycin associated protein 1
别名 FRAP1, MTOR, FRAP2, FRAP, FKBP12-rapamycin complex-associated protein
计算分子量 289 kDa
观测分子量250-289 kDa
GenBank蛋白编号BC117166
基因名称 mTOR
Gene ID (NCBI) 2475
RRIDAB_2918905
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481. mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors.