Phospho-mTOR (Ser2448) Recombinant antibody
Phospho-mTOR (Ser2448) Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, Rat and More (4)
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
3L18
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, Calyculin A treated HSC-T6 cells, HeLa cells, Calyculin A treated HEK-293 cells, Calyculin A treated HeLa cells |
Positive IF/ICC detected in | PMA treated HEK-293 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 22 publications below |
产品信息
80596-1-RR targets Phospho-mTOR (Ser2448) in WB, IF/ICC, ELISA applications and shows reactivity with Human, Rat samples.
Tested Applications | WB, IF/ICC, ELISA Application Description |
Cited Applications | WB |
Tested Reactivity | Human, Rat |
Cited Reactivity | human, mouse, rat, bovine |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | FK506 binding protein 12-rapamycin associated protein 1 |
Synonyms | FLJ44809, FRAP, FRAP1, FRAP2, Mammalian target of rapamycin, MTOR, Phospho-mTOR (Ser2448), RAFT1, Rapamycin target protein 1, RAPT1 |
Calculated Molecular Weight | 289 kDa |
Observed Molecular Weight | 250-289 kDa |
GenBank Accession Number | BC117166 |
Gene Symbol | mTOR |
Gene ID (NCBI) | 2475 |
RRID | AB_2918905 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for Phospho-mTOR (Ser2448) antibody 80596-1-RR | Download protocol |
IF protocol for Phospho-mTOR (Ser2448) antibody 80596-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Free Radic Biol Med β-Sitosterol targets ASS1 for Nrf2 ubiquitin-dependent degradation, inducing ROS-mediated apoptosis via the PTEN/PI3K/AKT signaling pathway in ovarian cancer | ||
J Affect Disord Prenatal alcohol exposure enhanced alcohol preference and susceptibility to PTSD in a sex-dependent manner through the synaptic HCN1 channel | ||
J Cell Mol Med Pan-cancer analysis of DCTN2 and its tumour-promoting role in HCC by modulating the AKT pathway | ||
Front Cell Dev Biol The Establishment of Quantitatively Regulating Expression Cassette with sgRNA Targeting BIRC5 to Elucidate the Synergistic Pathway of Survivin with P-Glycoprotein in Cancer Multi-Drug Resistance. | ||
J Ethnopharmacol Anti-apoptotic effect of HeidihuangWan in renal tubular epithelial cells via PI3K/Akt/mTOR signaling pathway |