Phospho-mTOR (Ser2448) Recombinant antibody
Phospho-mTOR (Ser2448) Recombinant Antibody for IF/ICC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, Rat and More (4)
Applications
IF/ICC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
3L18
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, Calyculin A treated HSC-T6 cells, HeLa cells, Calyculin A treated HEK-293 cells, Calyculin A treated HeLa cells |
Positive IF detected in | PMA treated HEK-293 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF) | IF : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 16 publications below |
产品信息
80596-1-RR targets Phospho-mTOR (Ser2448) in WB, IF, ELISA applications and shows reactivity with Human, Rat samples.
Tested Applications | IF/ICC, WB, ELISA |
Cited Applications | WB |
Tested Reactivity | Human, Rat |
Cited Reactivity | human, mouse, rat, bovine |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | FK506 binding protein 12-rapamycin associated protein 1 |
Synonyms | FLJ44809, FRAP, FRAP1, FRAP2, Mammalian target of rapamycin, MTOR, Phospho-mTOR (Ser2448), RAFT1, Rapamycin target protein 1, RAPT1 |
Calculated Molecular Weight | 289 kDa |
Observed Molecular Weight | 250-289 kDa |
GenBank Accession Number | BC117166 |
Gene Symbol | FRAP1 |
Gene ID (NCBI) | 2475 |
RRID | AB_2918905 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for Phospho-mTOR (Ser2448) antibody 80596-1-RR | Download protocol |
IF protocol for Phospho-mTOR (Ser2448) antibody 80596-1-RR | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
J Affect Disord Prenatal alcohol exposure enhanced alcohol preference and susceptibility to PTSD in a sex-dependent manner through the synaptic HCN1 channel | ||
Front Cell Dev Biol The Establishment of Quantitatively Regulating Expression Cassette with sgRNA Targeting BIRC5 to Elucidate the Synergistic Pathway of Survivin with P-Glycoprotein in Cancer Multi-Drug Resistance. | ||
J Ethnopharmacol Anti-apoptotic effect of HeidihuangWan in renal tubular epithelial cells via PI3K/Akt/mTOR signaling pathway | ||
J Biochem Mol Toxicol ATL1 inhibits the proliferation and invasion of trophoblast cells via inhibition of the mTOR signaling pathway | ||
Int J Ophthalmol Effects of Lycium barbarum polysaccharide on the photoinduced autophagy of retinal pigment epithelium cells. |