CoraLite® Plus 647-conjugated Phospho-mTOR (Ser2448) Recombinant antibody
Phospho-mTOR (Ser2448) Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
Human, Rat
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
3L18
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | Rapamycin treated HeLa cells |
| Positive FC (Intra) detected in | Calyculin A treated HeLa cells, HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy MTOR Ready-To-Use IHC Kit. MTOR primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 80596-1-PBS
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL647-80596 targets Phospho-mTOR (Ser2448) in IF/ICC, FC (Intra) applications and shows reactivity with Human, Rat samples.
| Tested Applications | IF/ICC, FC (Intra) Application Description |
| Tested Reactivity | Human, Rat |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | FK506 binding protein 12-rapamycin associated protein 1 |
| Synonyms | FRAP1, MTOR, FRAP2, FRAP, FKBP12-rapamycin complex-associated protein |
| Calculated Molecular Weight | 289 kDa |
| Observed Molecular Weight | 250-289 kDa |
| GenBank Accession Number | BC117166 |
| Gene Symbol | mTOR |
| Gene ID (NCBI) | 2475 |
| Conjugate | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481. mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 647 Phospho-mTOR (Ser2448) antibody CL647-80596 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |