验证数据展示
经过测试的应用
| Positive WB detected in | recombinant protein |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IP | See 1 publications below |
产品信息
66945-1-Ig targets Protein A in WB, IP, ELISA applications and shows reactivity with staphylococcus aureus samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IP |
| 经测试反应性 | staphylococcus aureus |
| 文献引用反应性 | human, mouse, yeast |
| 免疫原 | Recombinant Protein 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | Protein A |
| 别名 | |
| GenBank蛋白编号 | EU695225 |
| 基因名称 | |
| Gene ID (NCBI) | |
| RRID | AB_2882269 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protein A is a surface protein of S.aureus which binds IgG molecules by their Fc region (PMID: 4163007). Engineered Protein A has been widely used in antibody purification due to its Fc binding property. However, trace of protein A might be leaked from purification resin thus causes contamination of final products. Antibody against Protein A or ELISA kits for Protein A could be used to evaluate leaking level of Protein A from resin. Protein A should be deactivated (e.g. boiling in 5-10% tween-20) before testing.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Protein A antibody 66945-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Virus Res HA2 from the Helicoverpa armigera nucleopolyhedrovirus: a WASP-related protein that activates Arp2/3-induced actin filament formation. | ||
J Biochem Title: Cloaking the ACE2 receptor with salivary cationic proteins inhibits SARS-CoV-2 entry. | ||
Hepatology Concerted synergy between viral-specific IgG and CD8 + T cells is critical for clearance of an HCV-related rodent hepacivirus | ||
Proc Natl Acad Sci U S A DNA polymerase delta governs parental histone transfer to DNA replication lagging strand |