验证数据展示
发表文章中的应用
| WB | See 1 publications below |
产品信息
66945-1-PBS targets Protein A in WB, Indirect ELISA applications and shows reactivity with staphylococcus aureus samples.
| 经测试应用 | WB, Indirect ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | staphylococcus aureus |
| 文献引用反应性 | yeast |
| 免疫原 | Recombinant Protein 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | Protein A |
| 别名 | |
| GenBank蛋白编号 | EU695225 |
| 基因名称 | |
| Gene ID (NCBI) | |
| RRID | AB_2882269 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| 储存缓冲液 | PBS only , pH 7.3 |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
Protein A is a surface protein of S.aureus which binds IgG molecules by their Fc region (PMID: 4163007). Engineered Protein A has been widely used in antibody purification due to its Fc binding property. However, trace of protein A might be leaked from purification resin thus causes contamination of final products. Antibody against Protein A or ELISA kits for Protein A could be used to evaluate leaking level of Protein A from resin. Protein A should be deactivated (e.g. boiling in 5-10% tween-20) before testing.