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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated RAB11FIP2 Polyclonal antibody

RAB11FIP2 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-18136

Print datasheet

Synonyms

KIAA0941, nRip11, Rab11 FIP2, RAB11FIP2

验证数据展示

  • IF Staining of HepG2 using CL488-18136

    IF Staining of HepG2 using CL488-18136

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 RAB11FIP2 antibody (CL488-18136) at dilution of 1:200.

  • FC experiment of HeLa using CL488-18136

    FC experiment of HeLa using CL488-18136

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated RAB11FIP2 Polyclonal antibody (CL488-18136)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 18136-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-18136 targets RAB11FIP2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen RAB11FIP2 fusion protein Ag12736 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAB11 family interacting protein 2 (class I)
Synonyms KIAA0941, nRip11, Rab11 FIP2, RAB11FIP2
Calculated Molecular Weight 512 aa, 58 kDa
Observed Molecular Weight 58 kDa
GenBank Accession NumberBC075073
Gene Symbol RAB11FIP2
Gene ID (NCBI) 22841
RRIDAB_3672688
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7L804
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RAB11FIP2 antibody CL488-18136Download protocol
FC protocol for CL Plus 488 RAB11FIP2 antibody CL488-18136Download protocol
Standard Protocols
Click here to view our Standard Protocols